SD大鼠术后急性疼痛向慢性疼痛转化的差异代谢物分析

doi:10.11843/j.issn.0366-6964.2021.03.024

摘要/Abstract

摘要： 术后慢性疼痛病因十分复杂，迄今发生机制尚未明析。本研究旨在通过动物模型解析术后急性疼痛向慢性疼痛转化的相关物质，以期找到引发术后疼痛的关键物质。采用气相色谱-质谱（GC-MS）代谢组学技术，比较分析大鼠术后急性疼痛和慢性坐骨神经紧缩损伤疼痛的相关物质，筛选差异代谢物。结果经分析后得到224种代谢物，其中，35种代谢物具有显著差异（VIP>1，P<0.05，∣log₂FC∣≥2），代谢通路富集分析发现，45条潜在相关代谢通路（P<0.05）。分析表明，N-乙酰天冬氨酸（NAA）、泛酸、天冬氨酸、3-羟基丁酸、β-丙氨酸、葡萄糖等差异代谢物及其相关代谢通路可能与术后急性疼痛向慢性疼痛转变密切相关。解析出术后急性疼痛向慢性疼痛转化的相关物质，这为术后疼痛机制的研究提供了参考，也为术后疼痛的识别和治疗提供了新的方法和思路。

关键词: 术后急性疼痛, 术后慢性疼痛, 转化, 关键物质

Abstract: The mechanisms of chronic postsurgical pain (CPSP) are complicated and still eludes explanation. In this study, a surgery-related animal model in rat was used to search the metabolites on the transition from acute to chronic pain, aiming to identify the potential biomarkers associated postsurgical pain. GC-MS-based metabolomics technique were performed to investigate the different metabolites between acute to chronic postsurgical pain. It showed that 224 metabolites were screened, while 35 of which were significant difference (VIP>1,P<0.05,∣log₂FC∣ ≥ 2). These metabolites were mainly involved in 45 metabolic pathways through pathway enrichment analysis. The results showed that metabolites such as N-acetylaspartic acid, aspartic acid, pantothenic acid, beta-alanine, 3-hydroxybutyric acid and alanine/aspartate/glutamate metabolism,β-alanine metabolism, pantothenate and CoA biosynthesis, regulating sugar metabolic pathways are closely related with the transition from acute to chronic pain. To dissertate the key substances of the transition of acute pain changed into chronic pain can not only help us to understand underlying mechanisms of CPSP, but also provide a new insight and method for recognizing and managing postsurgical pain.

Key words: acute postsurgical pain, chronic postsurgical pain, transition, key substance

中图分类号:

S857.128

王国辽, 林辉, 吕俊瑾, 刘镇, 程娇娇, 唐雷雷, 莫睿文, 远立国. SD大鼠术后急性疼痛向慢性疼痛转化的差异代谢物分析[J]. 畜牧兽医学报, 2021, 52(3): 799-808.

WANG Guoliao, LIN Hui, LÜ Junjin, LIU Zhen, CHENG Jiaojiao, TANG Leilei, MO Ruiwen, YUAN Liguo. Analysis on the Difference of Metabolites of Transition from Acute Postsurgical Pain to Chronic Postsurgical Pain in SD Rat[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 799-808.

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