1株分离自云南省师宗县库蠓的新型西藏环状病毒

doi:10.11843/j.issn.0366-6964.2021.017

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (1): 166-176.doi: 10.11843/j.issn.0366-6964.2021.017

1株分离自云南省师宗县库蠓的新型西藏环状病毒

杨振兴¹, 谢佳芮¹, 李占鸿¹, 李卓然¹, 廖德芳¹, 牛保生², 姚萍芬², 李乐^1*, 杨恒^1*

1. 云南省畜牧兽医科学院云南省热带亚热带动物病毒病重点实验室, 昆明 650224;
2. 云南省师宗县动物疫病预防控制中心, 曲靖 655700

收稿日期:2020-04-28 出版日期:2021-01-23 发布日期:2021-01-19
通讯作者: 李乐,主要从事动物虫媒病毒研究,E-mail:lilesn@sina.com;杨恒,主要从事动物病毒学研究,E-mail:yangheng2008.cool@163.com
作者简介:杨振兴(1986-),男,云南昆明人,助理研究员,硕士,主要从事牛羊虫媒病毒研究,E-mail:s300yn@163.com;谢佳芮(1990-),女,云南昆明人,助理研究员,硕士,主要从事牛羊虫媒病毒研究及细胞培养,E-mail:349457530@qq.com。杨振兴和谢佳芮为同等贡献作者
基金资助:
国家重点研发计划（2016YFD0500908）；国家重点研发计划（2017YFC1200505）；国家公益性行业（农业）科研专项（201303035）；云南省中青年学术和技术带头人后备人才培养项目（2017HB055）

Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province

YANG Zhenxing¹, XIE Jiarui¹, LI Zhanhong¹, LI Zhuoran¹, LIAO Defang¹, NIU Baosheng², YAO Pingfen², LI Le^1*, YANG Heng^1*

1. Yunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Veterinary and Animal Science Institute, Kunming 650224, China;
2. Animal Disease Control Center of Shizong County, Qujing 655700, China

Received:2020-04-28 Online:2021-01-23 Published:2021-01-19

摘要/Abstract

摘要： 西藏环状病毒（Tibet orbivirus，TIBOV）为环状病毒属的新成员，病毒于2009年首次从我国西藏墨脱县采集的圆斑按蚊中分离，目前，对该病毒的认识仍十分有限。本研究对1株分离自我国云南省师宗县库蠓的新型TIBOV毒株YNSZ/V290/2019进行了鉴定，结果表明，病毒可在C6/36与BHK-21细胞上引起明显的细胞病变，电镜下观察，病毒粒子呈球形，直径55~75 nm，具有环状病毒属病毒典型形态特征。电泳结果显示，病毒基因组为十节段双链RNA，呈现"3-3-3-1"的电泳带型特征。对环状病毒属病毒高度保守的基因节段及其编码蛋白（Seg-3/T2）的序列分析显示，YNSZ/V290/2019为TIBOV的成员，与其他TIBOV毒株的核酸与氨基酸序列相似度分别为80.90%~81.70%与97.20%~97.30%。对决定环状病毒属病毒血清型的基因节段及其编码蛋白（Seg-2/OC1）的序列分析显示，YNSZ/V290/2019与已知TIBOV毒株的核酸与氨基酸序列相似度分别为26.90%~30.60%与28.50%~33.20%，表明该毒株可能是一种新血清型的TIBOV。对采集于师宗县牧场的40份黄牛与68份山羊血清进行血清中和试验，结果显示，新型TIBOV在牛、羊的阳性率分别为22.50%与4.41%。新型TIOBV在我国的发现，丰富了我国虫媒病毒的研究，为深入研究我国TIBOV的分布、致病性、感染宿主范围与诊断试剂的开发提供了基础。

关键词: 库蠓, 病毒分离鉴定, 西藏环状病毒, 序列分析

Abstract: Tibet orbivirus (TIBOV) is a new member of the genus Orbivirus. The virus was first isolated from Anopheles maculatus collected in Motuo County, Tibet, China in 2009. At present, the recognition of this virus is still very limited. This is the first report on the isolation and identification of a new TIBOV strain YNSZ/V290/2019 from Culicoides collected in Shizong County, Yunnan Province, China. YNSZ/V290/2019 can cause an obvious cytopathic effect on C6/36 and BHK-21 cells. Under the electron microscope, the virus particles are spherical, with a diameter of about 55-75 nm, and have typical morphological characteristics of cycloviruses. The results of agarose gel electrophoresis showed that the genome of the virus was a 10 segment double-stranded RNA, showing the characteristics of "3-3-3-1". The sequence analysis of the highly conserved gene (Seg-3) and proteins (T2) showed that YNSZ/V290/2019 was a member of TIBOV, and YNSZ/V290/2019 shared 80.90%-81.70% nucleic acid sequence identity and 97.20%-97.30% amino acid sequence identity with other TIBOV strains. The sequence analysis of the high variable gene (Seg-2) and proteins (OC1) which determine the serotype of the Orbivirus showed that YNSZ/V290/2019 shared 26.90%-30.60% nucleic acid sequence identity and 28.5%-33.2% amino acid sequence identity with the known TIBOV strains, suggested that the strain may represent a new serotype of TIBOV. Serum neutralization tests were carried out on 40 cattle and 68 goat serum samples collected from Shizong pasture. The results showed that the positive rates of new TIBOV of cattle and sheep were 22.50% and 4.41%, respectively. The discovery of a new type of TIOBV in China enriches the research on arbovirus in China and provides a basis for further study on the distribution, pathogenicity, and host range of infection and development of diagnostic reagents of TIBOV in China.

Key words: Culicoides, virus isolation and identification, Tibet orbivirus (TIBOV), sequence analysis

中图分类号:

S852.65

杨振兴, 谢佳芮, 李占鸿, 李卓然, 廖德芳, 牛保生, 姚萍芬, 李乐, 杨恒. 1株分离自云南省师宗县库蠓的新型西藏环状病毒[J]. 畜牧兽医学报, 2021, 52(1): 166-176.

YANG Zhenxing, XIE Jiarui, LI Zhanhong, LI Zhuoran, LIAO Defang, NIU Baosheng, YAO Pingfen, LI Le, YANG Heng. Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 166-176.

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1株分离自云南省师宗县库蠓的新型西藏环状病毒

Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province

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