烯丙孕素口服液在猪体内的残留消除研究

doi:10.11843/j.issn.0366-6964.2018.09.022

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (9): 2005-2014.doi: 10.11843/j.issn.0366-6964.2018.09.022

烯丙孕素口服液在猪体内的残留消除研究

王双^1,2, 杨海峰^1,2, 余波^1,3, 李士洋^1,3, 郑育基^1,3, 卜仕金^1,3*

1. 扬州大学兽医学院, 扬州 225009;
2. 江苏农牧科技职业学院, 泰州 225300;
3. 江苏省动物重要疫病与人畜共患病防控协同创新中心, 扬州 225009

收稿日期:2018-01-17 出版日期:2018-09-23 发布日期:2018-09-23
通讯作者: 卜仕金(1964-),男,安徽人,教授,主要从事兽医药理与毒理学研究,E-mail:pushijin@aliyun.com
作者简介:王双(1993-),女,河南人,硕士生,主要从事药理学研究,E-mail:1289656619@qq.com
基金资助:
江苏省普通高校研究生科研创新计划项目（SJLX15＿0675）；扬州大学“新世纪人才工程”资助项目

Study on Residue Elimination of Altrenogest Oral Solution in Pigs

WANG Shuang^1,2, YANG Hai-feng^1,2, YU Bo^1,3, LI Shi-yang^1,3, ZHENG Yu-ji^1,3, BU Shi-jin^1,3*

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;
3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infections Diseases and Zoonoses, Yangzhou 225009, China

Received:2018-01-17 Online:2018-09-23 Published:2018-09-23

摘要/Abstract

摘要：

本研究旨在分析烯丙孕素在猪体内的残留消除规律，为制定休药期提供依据。采集猪肝、肾、肌肉和脂肪样品，肝、肾样品经β-葡萄糖醛酸酶/芳基硫酸酯酶酶解，然后用乙腈提取（肌肉和脂肪样品直接用乙腈提取），乙腈饱和正己烷溶液除脂，氮吹后残渣用乙酸乙酯复溶，后用10%碳酸钠溶液净化进行LC-MS/MS分析，外标法定量。结果表明：在1~200 ng·mL^-1浓度范围内，烯丙孕素具有良好的线性关系，相关系数大于0.999，方法的检测限为0.5 μg·kg^-1，定量限为1 μg·kg^-1；在1、100、200 μg·kg^-1添加水平下，化合物的平均回收率在68.9%~78.9%，相对标准偏差均小于11%。试验猪按0.4 mg·kg^-1剂量内服给药，每天1次，连续给药18 d，经取材检测，休药6 h后各组织中烯丙孕素残留量最高，休药后第5天脂肪中烯丙孕素残留量低于最大残留限量，肌肉、肾中残留量低于定量限，休药第10天所有组织中烯丙孕素残留量均低于定量限。休药后相同时间点猪组织中烯丙孕素残留量规律为肝 > 肾 > 脂肪 > 肌肉；肝中烯丙孕素消除缓慢且消除时间最长。根据WT1.4软件按99%置信区间计算所得，建议母猪按0.4 mg·kg^-1剂量内服给药，每天1次，连续18 d，其休药期为9 d。

Abstract:

The experiment was conducted to study the residues of altrenogest in swine tissues, preparing for the determination of withdrawal time. The liver and kidney samples were hydrolyzed by β-glucosidase/arysulfatase and extracted by acetonitrile (the muscle and fat samples were extracted by acetonitrile), then the extracting solution were degreased with n-hexane. After being redissolved with ethyl acetate, the supernatant was purified by 10% sodium carbonate solution. Finally, altrenogest was analyzed by LC-MS/MS, and was calibrated by the external method. The results showed that the standard curve was obtained with the correlation coefficients (R²=0.999) in the concentration range of 1-200 ng·mL^-1. The limit of quantification for altrenogest was 1.0 μg·kg^-1, and the limit of detection for altrenogest was 0.5 μg·kg^-1. The recoveries of altrenogest were 68.9%-78.9% at three spiked levels of 1, 100, 200 μg·kg^-1 with the relative standard deviations less than 11% (n=6). After the pigs were orally administered altrenogest of 0.4 mg·kg^-1 of body weight onetime every day for 18 d, the concentration of in all of the tissues at 6 h was highest; the concentration of altrenogest in fat at 5 d was lower than MRL, while the concentration of altrenogest in muscle and kidney tissues were lower than LOQ; the concentration of altrenogest in all of the tissues at 10 d were lower than LOQ. The order of residue rate was liver > kidney > fat > muscle, and liver was considered as the target organ. The concentration-time dates were analyzed by WT1.4 to get the withdrawal time with 99% upper tolerance limit, the WT advised was 9 d.

中图分类号:

S859.84

王双, 杨海峰, 余波, 李士洋, 郑育基, 卜仕金. 烯丙孕素口服液在猪体内的残留消除研究[J]. 畜牧兽医学报, 2018, 49(9): 2005-2014.

WANG Shuang, YANG Hai-feng, YU Bo, LI Shi-yang, ZHENG Yu-ji, BU Shi-jin. Study on Residue Elimination of Altrenogest Oral Solution in Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2005-2014.

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烯丙孕素口服液在猪体内的残留消除研究

Study on Residue Elimination of Altrenogest Oral Solution in Pigs

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