中药成分辛弗林对胶体金免疫层析法检测β-受体激动剂的影响

doi:10.11843/j.issn.0366-6964.2021.012.034

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (12): 3675-3680.doi: 10.11843/j.issn.0366-6964.2021.012.034

中药成分辛弗林对胶体金免疫层析法检测β-受体激动剂的影响

王子琳¹, 唐子恒¹, 林泽宁¹, 刘文字², 刘戎², 张嘉慧², 沈祥广^2*

1. 华南农业大学国家兽药残留基准实验室, 广州 510642;
2. 农业农村部畜禽产品质量监督检验测试中心(广州), 广州 510642

收稿日期:2021-03-24 出版日期:2021-12-25 发布日期:2021-12-22
通讯作者: 沈祥广,主要从事动物性食品安全研究,E-mail:shenxg@scau.edu.cn
作者简介:王子琳(1995-),女,广东深圳人,硕士,主要从事动物性食品安全研究,E-mail:wzl3073075@stu.scau.edu.cn
基金资助:
2020年农业国家标准和行业标准制修订项目（SCB-30049）；广东省公益性研究与能力建设项目（2015A020209130）

Effect of Traditional Chinese Medicine Compound Synephrine on the Detection of β-agonists Using Colloidal Gold Immunochromatographic Assay

WANG Zilin¹, TANG Ziheng¹, LIN Zening¹, LIU Wenzi², LIU Rong², ZHANG Jiahui², SHEN Xiangguang^2*

1. National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, China;
2. Animal Poultry Products Quality Control, Inspection and Testing Center(Guangzhou), Ministry of Agriculture, Guangzhou 510642, China

Received:2021-03-24 Online:2021-12-25 Published:2021-12-22

摘要/Abstract

摘要： 旨在探究陈皮、青皮、木瓜、厚朴、红景天等5种中药材提取液以及饲喂了陈皮和青皮的猪尿液胶体金免疫层析法检测结果呈阳性的原因。建立测定猪尿及中药提取物中辛弗林的LC-MS/MS检测方法，测定陈皮、青皮的提取物和饲喂陈皮、青皮后猪尿中的辛弗林浓度。用小鼠肝微粒体孵育CGIA检测呈阳性的陈皮、青皮、木瓜和厚朴4味中药提取物。饲喂陈皮和青皮后的猪尿液检出辛弗林，浓度分别为1.36和1.65 μg·mL^-1；在陈皮和青皮的提取复溶液中检出辛弗林，浓度分别为132.6和312.7 μg·mL^-1。厚朴和木瓜两种中药提取物经过肝微粒体孵育后，在2~5 h逐渐由CGIA检测阳性转为阴性，陈皮和青皮孵育后，0~6 h的结果均为CGIA检测阳性；阴性中药组CGIA检测均呈阴性，而添加辛弗林的阳性对照组0~6 h CGIA检测均呈阳性。猪较大剂量使用陈皮和青皮会引起猪尿β-受体激动剂CGIA检测出现假阳性，该假阳性现象跟中药成分辛弗林有关，体外试验中辛弗林不易被小鼠肝微粒体代谢。

关键词: β-受体激动剂, 胶体金免疫层析法, 中药, 假阳性, 辛弗林

Abstract: This study was conducted to explore the reason why traditional Chinese medicine extractives and swine urines from pigs fed with some herbal were tested positive by CGIA. A LC-MS/MS method was established to determine synephrine in pigs' urine and traditional Chinese medicine extractives. Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium's extractives were determined, along with urine after herbal feed. In vitro mouse liver microsomes model was used to incubate the extractives of Citri Reticulatae Pericarpium Viride, Citri Reticulatae Pericarpium, Magnoliae Officinalis Cortex, and Chaenomelis Fructus. Synephrine was detected in pig urines after feeding with Citri Reticulatae Pericarpium and Citri Reticulatae Pericarpium Viride, and the concentration was 1.36 and 1.65 μg·mL^-1 respectively. The concentration of synephrine in Citri Reticulatae Pericarpium and Citri Reticulatae Pericarpium Viride's extractive was 132.6 and 312.7 μg·mL^-1 respectively. Extractives of Magnoliae Officinalis Cortex and Chaenomelis Fructus were tested gradually from positive to negative during 2-5 h incubation. Extractives of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium were tested positive during 0-6 h incubation. The experiment group of negative herbs were tested negative during incubation, and control group of synephrine matched were tested positive during 0-6 h incubation. Great dosing of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium in swine can cause false positive results of β-agonists'CGIA test in swine urine, and this phenomenon is related to synephrine. Synephrine is hard to be metabolized by mouse liver microsomes in vitro.

Key words: β-agonists, colloidal gold immunochromatographic assay, traditional Chinese medicine, false positive, synephrine

中图分类号:

S859.84

王子琳, 唐子恒, 林泽宁, 刘文字, 刘戎, 张嘉慧, 沈祥广. 中药成分辛弗林对胶体金免疫层析法检测β-受体激动剂的影响[J]. 畜牧兽医学报, 2021, 52(12): 3675-3680.

WANG Zilin, TANG Ziheng, LIN Zening, LIU Wenzi, LIU Rong, ZHANG Jiahui, SHEN Xiangguang. Effect of Traditional Chinese Medicine Compound Synephrine on the Detection of β-agonists Using Colloidal Gold Immunochromatographic Assay[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3675-3680.

参考文献 12

[1]	FEDDERN V, AROEIRA C N, MOLOGNONI L, et al. Ractopamine analysis in pig kidney, liver and lungs:a validation of the method scope extension using QuEChERS as a sample preparation step[J]. J Chromatogr B, 2018, 1091:79-86.
[2]	CHENG J, WANG S, ZHANG S, et al. Rapid and sensitive determination of clenbuterol residues in animal urine by surface-enhanced Raman spectroscopy[J]. Sensor Actuat B:Chem, 2019, 279:7-14.
[3]	ZHU C, ZHANG G L, HUANG Y F, et al. Aptamer based ultrasensitive determination of the β-adrenergic agonist ractopamine using PicoGreen as a fluorescent DNA probe[J]. Microchim Acta, 2017, 184(2):439-444.
[4]	HAKK H, SHELVER L W, CASEY F X M, et al. Fate and transport of the β-adrenergic agonist ractopamine hydrochloride in soil-water systems[J]. J Environ Sci, 2016, 45(7):40-48.
[5]	ZHANG Y, PENG J, GUO P, et al. Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay[J]. Food Agric Immunol, 2018, 29(1):711-721.
[6]	张琪, 李娜, 刘庆雨, 等. 日粮中添加杜仲粉对松辽黑猪生长性能、胴体性状、肉质性状及经济效益的影响[J]. 饲料工业, 2020, 41(23):34-38.ZHANG Q, LI N, LIU Q Y, et al. Effects of diets with Eucommia ulmoides powder adding on growth performance, carcass traits, meat quality traits and economic benefits of Songliao black pig[J]. Feed Industry, 2020, 41(23):34-38. (in Chinese)
[7]	易鑫, 林子清, 舒刚. 中药提高猪肉品质应用上的研究进展[J]. 猪业科学, 2021, 38(4):40-43.YI X, LIN Z Q, SHU G. Research progress on application of traditional Chinese medicine in improving pork quality[J]. Swine Industry Science, 2021, 38(4):40-43. (in Chinese)
[8]	任亚琪, 徐赓, 刘丽蓉, 等. 中药防治猪病毒性疫病研究进展[J]. 中国动物检疫, 2019, 36(5):49-56.REN Y Q, XU G, LIU L R, et al. Research progress on control of porcine viral diseases with traditional Chinese medicine[J]. China Animal Health Inspection, 2019, 36(5):49-56. (in Chinese)
[9]	朱买勋, 林渝宁, 周雪. 中药防治猪传染性胃肠炎作用研究进展[J]. 中国兽医学报, 2018, 38(6):1255-1259.ZHU M X, LIN Y N, ZHOU X. Research progress on the prevention and treatment of Chinese medicine on transmissible gastroenteritis[J]. Chinese Journal of Veterinary Science, 2018, 38(6):1255-1259. (in Chinese)
[10]	WANG Z L, FENG K Y, GE X F, et al. Effects of 105 traditional Chinese medicines on the detection of β-agonists in medicine extracts and swine urine based on colloidal gold immunochromatographic assay[J]. J Integr Agric, 2021, 20(6):1626-1635.
[11]	闫灵芝. 免疫层析试纸条技术在食品安全领域的研究进展[J]. 食品工业科技, 2021, 42(11):397-404, doi:10. 13386/j. issn1002-0306. 2020110079.YAN L Z. Research progress of immunochromatographic strip technique in food safety[J]. Science and Technology of Food Industry, 2021, 42(11):397-404, doi:10. 13386/j. issn1002-0306. 2020110079. (in Chinese)
[12]	WANG H L, YAO W F, ZHU D N, et al. Chemical fingerprinting by HPLC-DAD-ELSD and principal component analysis of Polygala japonica from different locations in China[J]. Chin J Nat Med, 2010, 8(5):343-348.

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中药成分辛弗林对胶体金免疫层析法检测β-受体激动剂的影响

Effect of Traditional Chinese Medicine Compound Synephrine on the Detection of β-agonists Using Colloidal Gold Immunochromatographic Assay

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