检测兔出血症病毒抗体胶体金试纸条的研制及初步应用

doi:10.11843/j.issn.0366-6964.2018.03.019

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (3): 614-619.doi: 10.11843/j.issn.0366-6964.2018.03.019

检测兔出血症病毒抗体胶体金试纸条的研制及初步应用

魏后军, 范志宇, 王芳*, 宋艳华, 胡波, 仇汝龙, 陈萌萌, 徐为中, 薛家宾

江苏省农业科学院兽医研究所·农业部动物疫病诊断与免疫重点开放实验室· 国家兽用生物制品工程技术研究中心, 南京 210014

收稿日期:2017-07-07 出版日期:2018-03-23 发布日期:2018-03-23
通讯作者: 王芳,研究员,E-mail:rwangfang@126.com
作者简介:魏后军(1986-),男,江苏南京人,本科,助理研究员,主要从事家兔疾病防治与兽医生物技术研究,Tel:025-84390337,E-mail:whj280941235@126.com
基金资助:
现代农业产业技术体系建设兔体系病毒病预防与控制岗位（CARS-44-C-1）；公益性行业（农业）科研专项（201303046）

Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody

WEI Hou-jun, FAN Zhi-yu, WANG Fang*, SONG Yan-hua, HU Bo, QIU Ru-long, CHEN Meng-meng, XU Wei-zhong, XUE Jia-bin

Key Laboratory of Veterinary Biologicals Engineering and Technology of Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2017-07-07 Online:2018-03-23 Published:2018-03-23

摘要/Abstract

摘要：

利用免疫层析技术建立快速检测兔出血症病毒（RHDV）抗体的方法，将胶体金标记重组RHDV VP60蛋白，抗VP60单抗A3C和金黄色葡萄球菌蛋白A（SPA）分别包被质控线（C线）和检测线（T线），通过检测强阳性、阳性、弱阳性、阴性血清优化试纸条制备条件，并验证其性能及与血凝抑制试验的符合率。结果显示，试纸条检测阳性血清时T线、C线颜色深度相同，强阳性血清T线比C线颜色深，弱阳性血清T线比C线颜色浅，阴性血清T线不显色、C线显色，检测强阳性、阳性、弱阳性、阴性血清与血凝抑制试验的符合率分别为93.75%、88.75%、91.25%、95.00%，总的符合率为91.54%；该试纸条具有较好的特异性、敏感性、重复性和稳定性，为兔病毒性出血症流行病学调查和抗体水平监测提供了有力的检测工具。

Abstract:

A colloidal gold test strip for rapid detection of RHDV antibody was developed based on colloidal gold immunochromatographic assay. The colloidal gold particles were labeled with recombinant RHDV VP60 protein. The anti-VP60 monoclonal antibody A3C and Staphylococcus aureus protein A (SPA) were respectively coated on the control line (C line) and test line (T line). The strip was optimized by detecting strong positive, positive, weak positive and negative serum, and its qualities and coincidence rate of HI test were verified. The results indicated that the T and C lines were the same color when the positive serum was detected. The T line was darker than the C line when the strong positive serum was detected. The T line was lighter than the C line when the weak positive serum was detected. Compared with HI test, the coincidence rates of strong positive, positive, weak positive, negative serum were 93.75%, 88.75%, 91.25%, 95.00%, respectively, while the total coincidence rate was 91.54%. The colloidal gold strip has specificity, sensitivity, repeatability and stability, and provide a tool for epidemiological survey and antibody level monitoring.

中图分类号:

魏后军, 范志宇, 王芳, 宋艳华, 胡波, 仇汝龙, 陈萌萌, 徐为中, 薛家宾. 检测兔出血症病毒抗体胶体金试纸条的研制及初步应用[J]. 畜牧兽医学报, 2018, 49(3): 614-619.

WEI Hou-jun, FAN Zhi-yu, WANG Fang, SONG Yan-hua, HU Bo, QIU Ru-long, CHEN Meng-meng, XU Wei-zhong, XUE Jia-bin. Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 614-619.

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检测兔出血症病毒抗体胶体金试纸条的研制及初步应用

Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody

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