陕西部分地区山羊毕氏肠微孢子虫多位点基因分型研究

doi:10.11843/j.issn.0366-6964.2017.09.019

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (9): 1730-1736.doi: 10.11843/j.issn.0366-6964.2017.09.019

陕西部分地区山羊毕氏肠微孢子虫多位点基因分型研究

刘婷丽¹, 彭先启³, 宋军科¹, 胡瑞思¹, 王莎莎¹, 潘广林^2*, 赵光辉^1*

1. 西北农林科技大学动物医学院, 杨凌 712100;
2. 陕西省珍稀野生动物抢救饲养研究中心, 周至 710402;
3. 河南牧翔动物药业有限公司, 郑州 450000

收稿日期:2017-03-23 出版日期:2017-09-23 发布日期:2017-09-23
通讯作者: 潘广林,E-mail:dongzi_1021@163.com;赵光辉,E-mail:zgh083@nwsuaf.edu.cn
作者简介:刘婷丽(1993-),女,山西吕梁人,硕士,主要从事动物寄生虫学与寄生虫病学的研究,E-mail:1229695951@qq.com;彭先启(1991-),男,河南周口人,硕士,主要从事兽医技术服务工作,E-mail:xianqi001@163.com。二人为共同第一作者

Multi-locus Sequence Genotyping Study of Enterocytozoon bieneusi in Goats from Partial Areas of Shaanxi Province

LIU Ting-li¹, PENG Xian-qi³, SONG Jun-ke¹, HU Rui-si¹, WANG Sha-sha¹, PAN Guang-lin^2*, ZHAO Guang-hui^1*

1. College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China;
2. Shaanxi Rare and Wildlife Rescuing and Breeding Center, Zhouzhi 710402, China;
3. Henan Muxiang Veterinary Pharmaceutical Co., Ltd, Zhengzhou 450000, China

Received:2017-03-23 Online:2017-09-23 Published:2017-09-23

摘要/Abstract

摘要：

为了阐明山羊毕氏肠微孢子虫的遗传多样性，笔者采用基于微卫星（MS1、MS3、MS7）和小卫星（MS4）位点的多位点序列分型技术首次对不同用途山羊的122个毕氏肠微孢子虫分离株进行了多位点序列分型研究。结果发现，在MS1、MS4和MS7位点的扩增效率依次为27.9%（34/122）、18.0%（22/122）、50.8%（62/122），而在MS3位点所有样品均未获得有效扩增。核苷酸序列分析表明，所有样品的MS1、MS4和MS7位点分别具有16、9和18个基因型，共形成了14个MLGs。其中，50份绒山羊阳性样品3个位点扩增效率分别为10.0%（5/50）、14.0%（7/50）和90.0%（45/50），分别具有3、3和10个基因型，共形成了5个MLGs；56份奶山羊阳性样品3个位点的扩增效率依次为28.6%（16/56）、19.6%（11/56）和8.9%（5/56），分别组成4、4、1个基因型，共组成7个不同的MLGs；16份黑山羊阳性样品在3个位点的扩增效率依次为81.3%（13/16）、25.0%（4/16）、75.0%（12/16），分别具有9、2、7个基因型，构成2个MLGs。

Abstract:

To indicate the genetic variability of Enterocytozoon bieneusi in goats, the multi-locus sequence genotypes (MLGs) of 122 E. bieneusi isolates from different production categories were studied using the multi-locus sequence genotyping (MLST) technique based on micro-(MS1, MS3, MS7) and mini-satellite (MS4) loci. The results showed that, the respective amplification efficiencies in loci MS1, MS4 and MS7 were 27.9%(34/122), 18.0%(22/122), 50.8%(62/122), while no amplifications were detected in the locus MS3. The nucleotide sequences analysis indicated that, 16, 9 and 18 genotypes were found in all positive samples for loci MS1, MS4 and MS7, respectively, forming fourteen MLGs. Among them, the amplification efficiencies of three loci among 50 positive samples of cashmere goats were 10.0% (5/50), 14.0% (7/50) and 90.0% (45/50), respectively, belonging to three, three and ten genotypes, and five MLGs. The amplification efficiencies of 56 positive samples of dairy goats in three loci were 28.6% (16/56), 19.6% (11/56) and 8.9% (5/56), grouping into four, four, one genotypes, and seven different MLGs. The amplification efficiencies of three loci of 16 positive samples of black goats were 81.3% (13/16) and 25.0% (4/16), 75.0% (12/16), clustering into 9, 2, 7 genotypes, and two MLGs.

中图分类号:

S852.723

刘婷丽, 彭先启, 宋军科, 胡瑞思, 王莎莎, 潘广林, 赵光辉. 陕西部分地区山羊毕氏肠微孢子虫多位点基因分型研究[J]. 畜牧兽医学报, 2017, 48(9): 1730-1736.

LIU Ting-li, PENG Xian-qi, SONG Jun-ke, HU Rui-si, WANG Sha-sha, PAN Guang-lin, ZHAO Guang-hui. Multi-locus Sequence Genotyping Study of Enterocytozoon bieneusi in Goats from Partial Areas of Shaanxi Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1730-1736.

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参考文献

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陕西部分地区山羊毕氏肠微孢子虫多位点基因分型研究

Multi-locus Sequence Genotyping Study of Enterocytozoon bieneusi in Goats from Partial Areas of Shaanxi Province

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