牦牛发情期卵巢比较转录组学研究

doi:10.11843/j.issn.0366-6964.2016.09.011

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (9): 1830-1839.doi: 10.11843/j.issn.0366-6964.2016.09.011

牦牛发情期卵巢比较转录组学研究

兰道亮^1，2，熊显荣²，柴志欣¹，艾鷖¹，黄偲²，李键^1，2*

（1.西南民族大学青藏高原研究院，成都 610041；2.西南民族大学生命科学与技术学院，成都 610041）

收稿日期:2016-03-31 出版日期:2016-09-23 发布日期:2016-09-23
通讯作者: 李键，教授，E-mail：lijian@swun.cn
作者简介:兰道亮(1984-)，男，畲族，江西德兴人，副研究员，博士，主要从事高原动物遗传育种研究，E-mail：landaoliang@163.com；Tel：028-85522552
基金资助:
西南民族大学中央高校基本科研业务费专项资金资助（2015NZYTD01）

Comparative Transcriptome Analysis between Yak and Cattle Estrus Ovary

LAN Dao-liang ^1，2，XIONG Xian-rong²，CHAI Zhi-xin ¹，AI Yi¹，HUANG Cai²，LI Jian ^1，2*

(1.Institute of Qinghai-Tibetan Plateau，Southwest University for Nationalities，Chengdu 610041，China；2.College of Life Science and Technology，Southwest University for Nationalities，Chengdu 610041， China)

Received:2016-03-31 Online:2016-09-23 Published:2016-09-23

摘要/Abstract

摘要：

旨在进一步了解牦牛发情期卵巢的分子机制，解析牦牛繁殖的特殊性。本研究应用RNA-seq技术对牦牛和平原黄牛发情期卵巢进行转录组高通量测序及全基因组差异表达模式比对分析。通过比较分析牦牛和黄牛卵巢转录组数据，共筛选出1 307个差异表达基因，其中661个基因表达量上调和646个基因表达量下调。进一步功能分析表明，这些差异基因涉及多种 GO分类及KEGG通路。其中GO分类注释显示，差异基因与细胞粘附、激素调控等生物学过程存在密切关联，同时钙离子结合、阳离子跨膜转运等分子事件表现活跃。KEGG通路分析显示，补体和凝血级联通路的富集水平最高，其次为细胞色素P450相关通路。昼夜节律等一些新型通路，尽管与生殖功能没有明显关联，但也表现出显著富集。本研究首次对比牦牛和黄牛发情期卵巢转录组数据，筛选并分析相关差异基因。该研究结果为进一步阐述牦牛卵巢的基本分子机理提供基础，同时也为全面理解牦牛繁殖特异性提供新的思路。

Abstract:

For elaborate the general physiological molecular features of the estrus ovary of yaks and particularity of yak reproduction.RNA-seq technology was applied to analyze transcriptome data comparatively between the yak and plain cattle estrus ovaries in this study.Compared with cattle，genome-wide divergent expression patterns during the ovary transcriptome data revealed that 1 307 genes were significantly and differentially expressed，in which 661 genes were upregulated and 646 genes were downregulated.Functional analysis showed that the differentially expressed genes were involved in various GO categories and KEGG pathways.GO annotations indicated that the genes were closely related to cell adhesion，hormonal biological processes，whereas the calcium ion binding，cation transmembrane transport molecular events were significantly active.KEGG pathway analysis showed that the complement and coagulation cascade pathway was the most enriched，followed by the cytochrome P450 related pathways.Moreover，several novel pathways，such as circadian rhythm，were significantly enriched despite having no evident associations with the reproductive function.This study is the first to compare the ovary transcriptome data between yak and cattle and to identify and analyze the related differential genes.Our findings provide a theory support for further investigation of the molecular mechanism of yak ovary and offer new insights to understand comprehensively the specificity of yak reproduction.

中图分类号:

S823.8+ 5.2

兰道亮，熊显荣，柴志欣，艾鷖，黄偲，李键. 牦牛发情期卵巢比较转录组学研究[J]. 畜牧兽医学报, 2016, 47(9): 1830-1839.

LAN Dao-liang，XIONG Xian-rong，CHAI Zhi-xin，AI Yi，HUANG Cai，LI Jian. Comparative Transcriptome Analysis between Yak and Cattle Estrus Ovary[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1830-1839.

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牦牛发情期卵巢比较转录组学研究

Comparative Transcriptome Analysis between Yak and Cattle Estrus Ovary

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