雏鸡感染鸡白痢沙门菌后禽β-防御素6和鸡Toll样受体15在肠道转录与定位研究

doi:10.11843/j.issn.0366-6964.2014.10.018

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (10): 1711-1717.doi: 10.11843/j.issn.0366-6964.2014.10.018

雏鸡感染鸡白痢沙门菌后禽β-防御素6和鸡Toll样受体15在肠道转录与定位研究

王燕¹，周秀红¹，祁克宗^1*，涂健¹，刘红梅¹，潘孝成²

（1.安徽农业大学动物科技学院，合肥230036；2.安徽省农业科学院，合肥 230031）

收稿日期:2014-02-24 出版日期:2014-10-23 发布日期:2014-10-23
通讯作者: 祁克宗，教授，E-mail：qkz@ahau.edu.cn
作者简介:王燕(1986-)，女，安徽安庆人，硕士，主要从事动物疫病病理与生物防治研究，E-mail：464309483@qq.com
基金资助:
863计划高新项目（2006AA10Z320）；国家自然科学基金（31372402）

Transcription and Positioning of Avian β-defensin 6 and Chicken TLR15 within the Intestinal Tract of Salmonella pullorum Infected Chicks

WANG Yan¹，ZHOU Xiu-hong¹，Qi Ke-zong^1*，TU Jian¹，LIU Hong-mei¹，PAN Xiao-cheng ²

(1.College of Animal Science and Technology，Anhui Agricultural University，Hefei 230036，China；2.Anhui Academy of Agricultural Sciences，Hefei 230031，China)

Received:2014-02-24 Online:2014-10-23 Published:2014-10-23

摘要/Abstract

摘要：

旨在分析雏鸡感染鸡白痢沙门菌后，禽β-防御素6 （AvBD6）和鸡Toll样受体15 （ChTLR15）在小肠内的分布及表达规律。本研究用鸡白痢沙门菌感染14日龄的雏鸡，感染后不同时间点采取雏鸡的小肠组织（十二指肠、空肠和回肠），利用实时荧光定量RT-PCR和原位杂交方法，检测AvBD6和ChTLR15在小肠组织内的定位和mRNA的转录水平变化。结果显示，雏鸡感染鸡白痢沙门菌后，AvBD6和ChTLR15在各肠段组织中均有转录，其中在十二指肠相对转录量最高，在0~24 h内呈上升趋势，24~48 h相对转录量呈下降趋势，在24 h的转录量极显著高于0和6 h (P<0.01)，且AvBD6在24 h的转录量显著高于12 h(P<0.05)。 AvBD6和ChTLR15 mRNA在小肠内均有不同程度的分布，其主要分布在肠绒毛的中央乳糜管中，在十二指肠中荧光信号最强。结果表明，雏鸡感染鸡白痢沙门菌后，AvBD6和ChTLR15 mRNA在鸡小肠中的分布和表达具有一定的规律性，并参与肠道免疫调节功能。

Abstract:

The current study was undertaken to analyze the distribution and expression of Avian β-defensin 6 (AvBD6) and chicken TLR15 (ChTLR15) in the small intestine of the chicks infected with Salmonella pullorum.Salmonella pullorum were used to infect chicks at the age of 14 days，and the small intestine (duodenum，jejunum and ileum) of the infected and control chicks were collected at different time points post infection.Fluorescence real-time quantitative PCR and in situ hybridization method were used to detect the positioning and transcription level changes of AvBD6 and ChTLR15 mRNA in the small intestine tissue.The results showed that after the infection of S.pullorum，AvBD6 and ChTLR15 were both transcribed in each bowel tissues of the chicks，and the relative transcription in the duodenum was the highest，the relative transcription levels showed a rising trend in the 0-24 h，the relative transcription levels decreased in 24-48 h，the level of AvBD6 and ChTLR15 transcription in 24 h was significantly higher than that of the o h and 6 h (P<0.01)，and the transcription of AvBD6 in 24 h was significantly higher than that of the 12 h (P<0.05).AvBD6 and ChTLR15 mRNA were distributed in the intestinal tissue，which were mainly distributed in the central lacteals of the intestinal villus，fluorescence signal in the duodenum was the strongest.These results indicated that chicks infected with S.pullorum，the transcription and distribution of AvBD6 and ChTLR15 mRNA in chicks’ intestine has certain regularity，and participate in the intestinal immune regulation function.

中图分类号:

王燕，周秀红，祁克宗，涂健，刘红梅，潘孝成. 雏鸡感染鸡白痢沙门菌后禽β-防御素6和鸡Toll样受体15在肠道转录与定位研究[J]. 畜牧兽医学报, 2014, 45(10): 1711-1717.

WANG Yan,ZHOU Xiu-hong,Qi Ke-zong，TU Jian，LIU Hong-mei，PAN Xiao-cheng . Transcription and Positioning of Avian β-defensin 6 and Chicken TLR15 within the Intestinal Tract of Salmonella pullorum Infected Chicks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1711-1717.

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雏鸡感染鸡白痢沙门菌后禽β-防御素6和鸡Toll样受体15在肠道转录与定位研究

Transcription and Positioning of Avian β-defensin 6 and Chicken TLR15 within the Intestinal Tract of Salmonella pullorum Infected Chicks

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