黄芩素对耐甲氧西林金黄色葡萄球菌抑菌机制的研究

doi:10.11843/j.issn.0366-6964.2013.12.021

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 2000-2006.doi: 10.11843/j.issn.0366-6964.2013.12.021

黄芩素对耐甲氧西林金黄色葡萄球菌抑菌机制的研究

陈禹先¹，周彤²，谢鲲鹏¹，云宝仪¹，谢明杰^1*

(1. 辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室，大连 116081；2. 阜新高等专科学校，阜新 123000)

收稿日期:2013-07-08 出版日期:2013-12-23 发布日期:2013-12-23
通讯作者: 谢明杰，教授，E-mail: xmj1222@sina.com
作者简介:陈禹先(1987-)，男，沈阳人，硕士研究生，主要从事微生物生化研究，E-mail: 375702508@qq.com
基金资助:
辽宁省教育厅科学研究一般项目（L2013412）；大连市科技计划项目(2013E13SF108)

Antibacterial Mechanism of Baicalein on Methicillin-resistant Staphyloccocus aureus

CHEN Yu-xian¹，ZHOU Tong²，XIE Kun-peng¹，YUN Bao-yi¹，XIE Ming-jie^1*

(1.Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, College of Life Science, Liaoning Normal University, Dalian 116081, China; 2.Fuxin College, Fuxin 123000, China)

Received:2013-07-08 Online:2013-12-23 Published:2013-12-23

摘要/Abstract

摘要：

研究黄芩素对耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌活性以及抑菌机制。以MRSA为供试菌，通过研究黄芩素对MRSA的抑制作用及其对细胞膜、蛋白质、拓扑异构酶活性的影响，并观察黄芩素与DNA结合后酶切图谱的变化等方面，较系统地阐述黄芩素对MRSA的抑菌作用机制。结果显示：黄芩素对MRSA有较强的抑制作用，其最小抑菌浓度为0.04 mg·mL^-1；黄芩素能抑制MRSA菌体蛋白质的合成，当黄芩素作用MRSA 16 h后，其菌体蛋白质的含量比对照组降低44.62%；黄芩素能抑制拓扑异构酶Ⅰ和Ⅱ的活性，当黄芩素的浓度为0.025 mg·mL^-1时即可抑制MRSA拓扑异构酶的活性；当黄芩素与DNA作用一定时间后，TaqⅠ的酶切电泳图谱发生了明显的变化，初步推测黄芩素与DNA的结合位点可能是在T与CGA碱基之间或结合在与T/CGA相类似的碱基位置。上述结果表明，黄芩素对MRSA有显著的抑菌活性，其作用机制可能是通过与DNA发生嵌入作用，抑制DNA拓扑异构酶的活性，使其不能进行正常的复制与转录，进而抑制了下游蛋白质的翻译过程，引起菌体内生物学功能的丧失，从而抑制菌体的生长和繁殖。

Abstract:

The study objective was to investigate the antibacterial activity and antibacterial mechanism of baicalein on Methicillin-resistant Staphylococcus aureus (MRSA). The experiments were carried out by studying baicalein inhibition against MRSA, determining the changes of membrane penetrability, protein synthesis, DNA topoisomerase activities and restriction map. Baicalein has a strong impact on MRSA, and the minimum inhibitory concentration reached 0.04 mg·mL^-1. After treated with Baicalein for 16 h, the total soluble content of proteins was decreased by 44.62%. Moreover, the activities of DNA topoisomerase Ⅰ and Ⅱ were inhibited by 0.025 mg·mL^-1 Baicalein. TaqⅠ restriction enzyme electropherogram appeared to be an obvious change when baicalein was added with DNA after a certain time. We initially speculated Baicalein with DNA binding sites may be between the bases T and CGA or be the T/CGA similar nucleotide positions. The results suggest that Baicalein may be embedded in bacterial DNA molecules, inhibits the activity of DNA topoisomerase and influences the normal DNA replication and transcription, thereby inhibiting protein translation in the downstream process, causing the loss of the bacteria biological function and inhibiting cell growth and reproduction.

中图分类号:

R285.5

陈禹先，周彤，谢鲲鹏，云宝仪，谢明杰. 黄芩素对耐甲氧西林金黄色葡萄球菌抑菌机制的研究[J]. 畜牧兽医学报, 2013, 44(12): 2000-2006.

CHEN Yu-xian，ZHOU Tong，XIE Kun-peng，YUN Bao-yi，XIE Ming-jie. Antibacterial Mechanism of Baicalein on Methicillin-resistant Staphyloccocus aureus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 2000-2006.

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黄芩素对耐甲氧西林金黄色葡萄球菌抑菌机制的研究

Antibacterial Mechanism of Baicalein on Methicillin-resistant Staphyloccocus aureus

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