内皮素-3对羊驼黑色素细胞特征及细胞内毛色基因表达的影响

doi:10.11843/j.issn.0366-6964.2013.07.012

摘要/Abstract

摘要：

为了研究内皮素-3（Endothelin-3，EDN3）对羊驼皮肤黑色素细胞内毛色形成相关基因的影响。本研究在体外培养的羊驼皮肤黑色素细胞中添加不同浓度（0、10^-9、10^-8、10^-7 mol·L^-1）的EDN3，通过MTT法、紫外分光光度法、qRT-PCR和Western blotting技术分别检测黑色素细胞活力、黑色素产量、相关基因和蛋白（包括内皮素受体B（Endothelin receptor B，EDNRB）、KIT、小眼畸形相关转录因子（Microphthalmia-associtated transcription factor, MITF）和酪氨酸酶（Tyrosinase, TYR））的表达情况。结果表明，在羊驼皮肤黑色素细胞添加EDN3 72 h后，黑色素细胞呈双树突或三树突状，且细胞数量明显增加；在添加适当浓度10^-8mol·L^-1时，细胞具有明显的增殖，细胞内EDNRB、KIT、MITF和TRY在转录水平和蛋白水平的表达量被上调，黑色素细胞产生黑色素的量也被上调，且与对照组相比，差异显著（P<0.05）。EDN3对羊驼黑色素细胞内黑色素的产生具有重要的调节作用。

Abstract:

The study aimed to investigate the effects of endothelin-3 (EDN3) on the expression of hair color genes of alpaca. The activity of melanocyte, the production of melanin in melanocytes and the expressions of related genes and proteins including Endothelin receptor B (EDNRB), KIT, Microphthalmia-associtated transcription factor (MITF) and Tyrosinase (TYR) in alpaca melanocytes in cultures with addition of different dose of EDN3（0, 10^-9, 10^-8, 10^-7 mol·L^-1）, were examined by MTT, ultraviolet spectrophotometry, qRT-PCR and Western blotting, respectively. The results showed that the amount of melanocytes was increased with the increasing double- or tri- dendrites; At the addition dose of 10^-8 mol·L^-1 for 72 h after adding EDN3, compared to control group, the proliferation of melanocytes was obvious and the expressions of EDNRB, KIT, MITF and TYR significantly up-regulated at the level of both transcription and translation in melanocytes（P<0.05）. Moreover, the production of melanin in melanocytes significantly increased（P<0.05）. These findings indicate that EDN3 play an important role in regulating melanins production in melanocytes in alpaca.

中图分类号:

S813.3

耿建军，白俊明，范瑞文，杨姗姗，庞亚妙，董常生. 内皮素-3对羊驼黑色素细胞特征及细胞内毛色基因表达的影响[J]. 畜牧兽医学报, 2013, 44(7): 1070-1077.

GENG Jian-jun, BAI Jun-ming, FAN Rui-wen, YANG Shan-shan, PANG Ya-miao, DONG Chang-sheng. The Effects of Endothelin-3 on the Melanocyte Characteristic and Expression of Hair Color Genes in Melanocytes from Alpaca (Lama Pacos) in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1070-1077.

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