顶复门原虫钙依赖蛋白激酶的研究进展

畜牧兽医学报

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顶复门原虫钙依赖蛋白激酶的研究进展

张念章¹，陈佳^1,2，王萌¹，朱兴全¹, 黄思扬^1*

（1. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室，兰州 730046； 2. 华南农业大学兽医学院，广州 510642）

收稿日期:2012-07-22 出版日期:2013-01-23 发布日期:2013-01-23
通讯作者: 黄思扬，Tel：0931-8342813，E-mail：hsy37@yahoo.cn
作者简介:张念章（1985-），男，山东德州人，博士生，主要从事寄生虫分子生物学方面的研究，E-mail：nianzhang2008@yahoo.com.cn
基金资助:
国家自然科学基金项目（31230073；31172316；31101812）；农业部农业科研杰出人才项目；中央级公益性科研院所基本科研业务费专项（2012ZL081）；中国农业科学院院长基金项目

Research Advances in Calcium-dependent Protein Kinases of Apicomplexa

ZHANG Nian-zhang¹, CHEN Jia^{1, 2}, WANG Meng¹, ZHU Xing-quan¹, HUANG Si-yang^1*

(1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China； 2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:2012-07-22 Online:2013-01-23 Published:2013-01-23

摘要/Abstract

摘要：

钙依赖蛋白激酶（Calcium-dependent protein kinases，CDPKs）是一类大的蛋白激酶家族，属于丝氨酸/苏氨酸类蛋白激酶，广泛存在于各种植物和原生动物中，参与多种生命活动的调控。随着生物信息学、分子生物学及基因工程的迅速发展，对顶复门原虫CDPKs的研究日益增多。研究结果表明，这类蛋白家族成员参与调控寄生虫入侵、外出宿主细胞、配子形成、宿主体内移行等顶复门原虫生活史的多个重要时期，其特殊的分子结构或可成为研究抗寄生虫疫苗或药物的候选靶标。本文以疟原虫、弓形虫和艾美耳球虫CDPKs为重点，综述了顶复门原虫CDPKs的结构、功能及生物学意义，展望了顶复门原虫CDPKs的研究和应用前景，以期为研究顶复门原虫的致病机理及研发抗原虫生物制剂提供参考。

Abstract:

Calcium-dependent protein kinases (CDPKs) are a large gene family, which is one kind of serine-threonine protein kinases. CDPKs have a diverse array of functions in various plants and many protozoa. With the development of bioinformatics, molecular biology and gene engineering, increasing numbers of researches about CDPKs were published recently. Increasing evidence suggests that CDPKs can regulate many crucial steps of parasite’s life cycle including host cell invasion and egress, gamete formation, and gliding motility. The special structure compared with proteins in human and mammal makes CDPKs an ideal target for novel vaccine and anti-parasite therapeutic drugs. This article reviews the structures, functions and applications of Apicomplexa’s CDPKs focusing on Plasmodium, Toxoplasma gondii and Eimeria, and prospects the research and application tendency, in order to provide references for the research of the pathogenesis and biological agents against Apicomplexan parasites.

中图分类号:

S852.72

张念章，陈佳，王萌，朱兴全, 黄思扬. 顶复门原虫钙依赖蛋白激酶的研究进展[J]. 畜牧兽医学报, doi: .

ZHANG Nian-zhang, CHEN Jia, WANG Meng, ZHU Xing-quan,HUANG Si-yang. Research Advances in Calcium-dependent Protein Kinases of Apicomplexa[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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参考文献

［1］HARMON A C, PUTNAM-EVANS C, CORMIER M J. A calcium-dependent but calmodulin- independent protein kinase from soybean ［J］. Plant Physiol, 1987, 83(4): 830-837.

［2］HARPER J F, SUSSMAN M R, SCHALLER G E, et al. A calcium-dependent protein kinase with a regulatory domain similar to calmodulin ［J］. Science, 1991, 252(5008): 951-954.

［3］YUASA T, MUTO S. Ca²⁺-dependent protein kinase from the halotolerant green alga Dunaliella tertiolecta: partial purification and Ca²⁺-dependent association of the enzyme to the microsomes ［J］. Arch Biochem Biophys, 1992, 296(1): 175-182.

［4］畅文君. 番茄钙依赖性蛋白激酶的生化鉴定和功能分析［D］. 海南:海南大学,2010.

［5］HARMON A C, GRIBSKOV M, HARPER J F. CDPKs - a kinase for every Ca²⁺ signal? ［J］. Trends Plant Sci, 2000, 5(4): 154-159.

［6］BILLKER O, LOURIDO S, SIBLEY L D. Calcium-dependent signaling and kinases in apicomplexan parasites ［J］. Cell Host Microbe, 2009, 5(6): 612-622.

［7］WERNIMONT A K, AMANI M, QIU W, et al. Structures of parasitic CDPK domains point to a common mechanism of activation ［J］. Proteins, 2011, 79(3): 803-820.

［8］LIM D C, COOKE B M, DOERIG C, et al. Toxoplasma and Plasmodium protein kinases: Roles in invasion and host cell remodeling ［J］. Int J Parasitol, 2012, 42(1): 21-32.

［9］KIESCHNICK H, WAKEFIELD T, NARDUCCI C A, et al. Toxoplasma gondii attachment to host cells is regulated by a calmodulin-like domain protein kinase ［J］. J Biol Chem, 2001, 276(15): 12369-12377.

［10］SUQI T, KATO K, KOBAYASHI K, et al. Molecular analyses of Toxoplasma gondii calmodulin-like domain protein kinase isoform 3 ［J］. Parasitol Int, 2009, 58(4): 416-423.

［11］TZEN M, BENAROUS R, DUPOUY-CAMET J, et al. A novel Toxoplasma gondii calcium-dependent protein kinase ［J］. Parasite, 2007, 14(2): 141-147.

［12］DOBROWOLSKI J M, CARRUTHERS V B, SIBLEY L D. Participation of myosin in gliding motility and host cell invasion by Toxoplasma gondii ［J］. Mol Microbiol, 1997, 26(1): 163-173.

［13］DONALD R G, ZHONG T, WIERSMA H, et al. Anticoccidial kinase inhibitors: identification of protein kinase targets secondary to cGMP-dependent protein kinase ［J］. Mol Biochem Parasitol, 2006, 49(1): 86-98.

［14］LOURIDO S, SHUMAN J, ZHANG C, et al. Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma ［J］. Nature, 2010, 465(7296): 359-362.

［15］HOLDER A A, MOHD RIDZUAN M A, GREEN J L. Calcium dependent protein kinase 1 and calcium fluxes in the malaria parasite ［J］. Microbes Infect, 2012, 14(10): 825-830.

［16］KUGELSTADT D, WINTER D, PLCKHAHN K, et al. Raf kinase inhibitor protein affects activity of Plasmodium falciparum calcium-dependent protein kinase 1 ［J］. Mol Biochem Parasitol, 2007, 151(1): 111-117.

［17］GREEN J L, REES-CHANNER R R, HOWELL S A, et al. The motor complex of Plasmodium falciparum: phosphorylation by a calcium-dependent protein kinase ［J］. J Biol Chem, 2008, 283(45): 30980-30989.

［18］KATO N, SAKATA T, BRETON G, et al. Gene expression signatures and small-molecule compounds link a protein kinase to Plasmodium falciparum motility ［J］. Nat Chem Biol, 2008, 4(6): 347-356.

［19］RIDZUAN M A, MOON R W, KNUEPFER E, et al. Subcellular location, phosphorylation and assembly into the motor complex of GAP45 during Plasmodium falciparum schizont development ［J］. PLoS One, 2012, 7(3): e 33854.

［20］SIDEN-KIAMOS I, ECKER A, NYBCK S, et al. Plasmodium berghei calcium-dependent protein kinase 3 is required for ookinete gliding motility and mosquito midgut invasion ［J］. Mol Microbiol, 2006, 60(6): 1355-1363.

［21］ISHINO T, ORITO Y, CHINZEI Y, et al. A calcium-dependent protein kinase regulates Plasmodium ookinete access to the midgut epithelial cell ［J］. Mol Microbiol, 2006, 59(4): 1175-1184.

［22］KATO K, SUDO A, KOBAYASHI K, et al. Characterization of Plasmodium falciparum calcium-dependent protein kinase 4 ［J］. Parasitol Int, 2009, 58(4): 394-400.

［23］BILLKER O, DECHAMPS S, TEWARI R, et al. Calcium and a Calcium-dependent protein kinase regulate gamete formation and mosquito transmission in a malaria parasite ［J］. Cell, 2004, 117: 503-514.

［24］DVORIN J D, MARTYN D C, PATEL S D, et al. A plant-like kinase in Plasmodium falciparum regulates parasite egress from erythrocytes ［J］. Science, 2010, 328(5980): 910-912.

［25］COPPI A, TEWARI R, BISHOP J R, et al. Heparan sulfate proteoglycans provide a signal to Plasmodium sporozoites to stop migrating and productively invade cells ［J］. Cell Host Microbe, 2007, 2(5): 316-327.

［26］周建民，鲍杰，汪明. 柔嫩艾美耳球虫甘肃株钙调域蛋白激酶基因的克隆与表达［J］. 畜牧兽医学报,2005,36(7):711-714.

［27］孔春林，韩红玉，李洋，等. 柔嫩艾美耳球虫钙依赖蛋白激酶3基因在毕赤酵母中的表达及分析［J］. 生物技术通报,2012,4:108-112.

［28］李洋. 柔嫩艾美耳球虫钙依赖蛋白激酶的研究［D］. 北京:中国农业科学院,2010.

［29］BUMSTEAD J M, TOMLEY F M, DUNN P P. Nitrocellulose immunoblotting for identification and molecular gene cloning of Eimeria maxima antigens that stimulate lymphocyte proliferation ［J］. Clin Diagn Lab Immunol, 1995, 2(5):86-98.

［30］DUNN P P, BUMSTEAD J M, TOMLEY F M. Sequence, expression and localization of calmodulin-domain protein kinases in Eimeria tenella and Eimeria maxima ［J］. Parasitology, 1996, 113(Pt5): 439-448.

［31］CAVAGNINO A, ROSSI F, RIZZI M. The potent antiplasmodial calmodulin-antagonist trifluoperazine inhibits Plasmodium falciparum calcium-dependent protein kinase 4 ［J］. Protein Peptide Lett, 2011, 18(12):1273-1279.

［32］OJO K K, LARSON E T, KEYLOUN K R, et al. Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors ［J］. Nat Struct Mol Biol, 2010, 17(5): 602-607.

［33］周东辉. 不同基因型弓形虫的 microRNA、蛋白质组及其感染宿主的蛋白质组比较研究［D］ . 广州:华南农业大学,2011.

［34］NAQAMUNE K, SIBLEY L D. Comparative genomic and phylogenetic analyses of calcium ATPases and calcium-regulated proteins in the apicomplexa ［J］. Mol Biol Evol, 2006, 23(8): 1613-1627.

［35］戚南山，孙铭飞，廖申权，等. 顶复门原虫入侵相关因子的研究进展［J］. 畜牧兽医学报,2012,43(2):167-174.

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顶复门原虫钙依赖蛋白激酶的研究进展

Research Advances in Calcium-dependent Protein Kinases of Apicomplexa

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