黄连解毒汤对感染大肠埃希菌大鼠脑组织TLR9表达的影响

畜牧兽医学报

黄连解毒汤对感染大肠埃希菌大鼠脑组织TLR9表达的影响

刘晓强，周宏超^*，俞亚玲，赵守中，杨鸣琦，李引乾

（西北农林科技大学动物医学院，杨凌712100）

收稿日期:2012-06-13 出版日期:2012-12-26 发布日期:2012-12-26
通讯作者: 周宏超，E-mail:zhcxn@126.com
作者简介:刘晓强（1977-），男，陕西富平人，讲师，博士生，主要从事临床兽医学研究，Tel：029-87091117，E-mail: liuxiaoqiang142@163.com
基金资助:
陕西省科技攻关项目（2009K02-01）

Effects of HuangLianJieDu Decoction on the Expression of TLR9 in Brain Tissue of Rats Infected with Escherichia coli

LIU Xiao-qiang, ZHOU Hong-chao^*, YU Ya-ling, ZHAO Shou-zhong, YANG Ming-qi, LI Yin-qian

(College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China)

Received:2012-06-13 Online:2012-12-26 Published:2012-12-26

摘要/Abstract

摘要：

为探讨Toll 样受体9 (TLR9) 及相关炎症因子在脑损伤中的调节机制，采用免疫组织化学SP法对正常大鼠、感染大肠埃希菌大鼠和黄连解毒汤预防大鼠脑组织中的TLR9表达及相关因子的动态变化进行了研究。66只SD大鼠随机分为正常对照组（n=6，腹腔注射生理盐水0.5 mL·只^-1）、试验组［n=30，腹腔注射大肠埃希菌菌液0.5 mL·只^-1（2.4×10⁹ CFU· mL^-1）］、黄连解毒汤预防组［n=30，每只按12.6 mL·kg^-1剂量提前6 d灌服黄连解毒汤，处理当天再分别腹腔注射大肠埃希菌菌液0.5 mL·只^-1（2.4×10⁹CFU· mL^-1）］。除对照组外，试验组和预防组又分6个小组，每小组5只大鼠。分别于处理后的3、6、9、12、18、24 h随机处死1小组试验组和预防组大鼠及1只对照组大鼠，免疫组化观察脑组织TLR9及相关因子的表达）。结果发现，TLR9在大鼠正常生理状态下有少量表达，感染大肠埃希菌后，TLR9的表达出现明显变化，且这种变化随着时间的推移而越发明显。在感染后3 h时TLR9表达量较正常生理状态下显著增加（P＜0.05），在6 h时开始显著增加，12 h时达到高峰，与对照组和试验组相比，均有极显著差异（P＜0.01）。预防组与对照组比较无统计学意义（P＞0.05）。该研究结果显示黄连解毒汤可通过抑制TLR9的表达对细菌所致的脑损伤产生保护作用。

Abstract:

The aim of this study was to investigate the mediation mechanism of toll-like receptor 9 (TLR9) and the related inflammatory factors in brain injury. Immunohistochemical ultra sensitive SP method was used to examine the dynamic expression of TLR9 and the related factors in the normal rats, injected with E. coli and HuangLianJieDu Decoction preventive rats in the present study. 66 SD rats were randomly divided into three groups. The control group (n=6) was treated with normal saline by intraperitoneal injection. Experimental group (n= 30) was treated with E. coli culture by intraperitoneal injection 0.5 mL per rat (2.4×10⁹ CFU· mL^-1). HuangLianJieDu Decoction preventive groups (n=30) was treated with 12.6 mL·kg^-1orally in advance 6 days, and then were intraperitoneally injected with 0.5 mL (2.4×10⁹CFU· mL^-1) per rat E. coli culture. Each group was divided into 6 sub-groups and each of which has 5 rats. The rats of each experimental group, prevention group and one rat in control group were killed randomly at 3, 6, 9, 12, 18, 24 hours after treated with E. coli. And then the expression of TLR9 and the related factors in brain were analyzed by immunohistochemistry. The results showed that there was a little amount of TLR9 expression in the brain under normal circumstances, while the TLR9 expression showed a significant change after E. coli infection, and it was more obvious over time. Compared with control group and prevention group, the expression of TLR9 increased significantly after 3 hours infected with E. coli, and it reached the peak after 12 hours (P＜0.01). There was no significant difference between prevention and control group (P＞0.05). These results indicated that the HuangLianJieDu Decoction can protect the brain injury by bacterial infection by suppressing the expression of TLR9.

中图分类号:

刘晓强，周宏超，俞亚玲，赵守中，杨鸣琦，李引乾. 黄连解毒汤对感染大肠埃希菌大鼠脑组织TLR9表达的影响[J]. 畜牧兽医学报, doi: .

LIU Xiao-qiang, ZHOU Hong-chao, YU Ya-ling, ZHAO Shou-zhong, YANG Ming-qi, LI Yin-qian. Effects of HuangLianJieDu Decoction on the Expression of TLR9 in Brain Tissue of Rats Infected with Escherichia coli[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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