Abstract:

 This experiment was conducted to study the change of the activity of NOS and the content of NO, and the influence of Xuesaitong and L-NNA on the change in Striatal Hemorrhage rabbits, and to investigate the mechanism of action of NO on the cerebral hemorrhage and the protective effect of those two pharmaceuticals on the cerebullar neurons. Fifty-six healthy Haerbin white rabbits, 4 moon's old, were divided into the model group, control group of sham operation and the treatment group of striatal hemorrhage rabbits with Xuesaitong and L-NNA injection in random. The mode of cerebral hemorrhage rabbits were established in this surgery , we determined the activity of NOS and the content of NO in different time after striatal hemorrhage in hemorrhagic corpus striatum by biochemical technology. The results indicated that the activity of NOS increased at 6 hours after striatal hemorrhage, and increased to the highest at 3 days, and then decreased gradually from 3 to 9 days, and basically returned to normal levels at 9 days. The activity of NOS in the two therapy groups were lower than that in model grope at every time extremely notable difference or notable difference (P<0.01 or P<0.05), and the level of decreasing the activity of NOS in the group of L-NNA was lower than that in the group of Xuesaitong between 6 hours to 1 day (P<0.05). The change of the content of NO and the activity of the NOS was accordant primitively and direct correlation. SABC immunohistochemical method was used to detected the neuronal NOS positive neurons in each corpus striatum. We found that the density of striatal neuronal NOS positive neurons increased, cell coloring became saturate, soma-sectional area and the length of longest tuber became smaller. The density of neuron reduced, and the soma-sectional area and the length of longest tuber ameliorated significantly after treatment (P<0.05). The results suggested that NO work in the process of cerebral hemorrhage damage, and the high concentrations of No play a neurotoxicity role in damage to brain tissue. Xuesaitong and L-NNA reduce the content of NO in the corpus striatum by inhibiting the activity of NOS, which has a significant protective effect on neurons in cerebral hemorrhage rabbits.