蛋鸡J亚群禽白血病病毒3′非编码区序列特征分析

畜牧兽医学报

蛋鸡J亚群禽白血病病毒3′非编码区序列特征分析

高玉龙，贠炳岭，秦立廷，潘伟，王永强，高宏雷，祁小乐，王笑梅^*

（中国农业科学院哈尔滨兽医研究所，兽医生物技术国家重点实验室，禽传染病研究室，哈尔滨 150001）

收稿日期:2012-04-23 出版日期:2012-11-26 发布日期:2012-11-26
通讯作者: 王笑梅，研究员，E-mail：xmw@hvri.ac.cn
作者简介:高玉龙（1974-），博士，副研究员，研究生导师，主要从事禽免疫抑制病研究，E-mail: ylg@hvri.ac.cn
基金资助:
国家自然科学基金（31072146）；现代农业肉鸡产业技术体系建设（nycytx-42-G3-01）；哈尔滨市科技攻关计划项目（2010AA6AN034）

Molecular Characteristics of 3′ Untranslated Regions of Avian Leukosis Virus Subgroup J in Layer Flocks

GAO Yu-long, YUN Bing-ling, QIN Li-ting, PAN Wei, WANG Yong-qiang, GAO Hong-lei, QI Xiao-le, WANG Xiao-mei^*

(Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China)

Received:2012-04-23 Online:2012-11-26 Published:2012-11-26

摘要/Abstract

摘要： 蛋用型鸡虽在试验条件下可以感染ALV-J，但自然感染时很少引起发病。然而，近年来在中国蛋鸡群中ALV-J发病严重，本研究对2008年以来ALV-J蛋鸡分离株的3′非编码区（3′UTR）进行了系统的分析。结果表明，89.5%（17/19）的ALV-J蛋鸡分离株的3′UTR出现了205 bp的缺失，94.7%（16/17）ALV-J蛋鸡分离株保留了完整的E元件。84.2%（16/19）的ALV-J蛋鸡分离株的U3区序列与肉鸡分离株的相似性低于92.5%，所有转录调控元件高度保守。ALV-J蛋鸡分离株的E元件和U3区均出现了多个碱基的突变。这些结果表明，ALV-J蛋鸡分离株的E元件和U3区正在迅速的演化，明显不同于肉鸡分离株的序列特征。这些发现有助于更好地了解ALV-J引起蛋鸡群发病的致病机理。

Abstract: Avian leukosis virus subgroup J (ALV-J) was first isolated from meat-type chickens in 1988. No field cases of ALV-J infection and tumors in layer chickens were observed worldwide until 2004. However, layer flocks in China have experienced outbreaks of this virus in recent years. The molecular epidemiology of 3′UTR of ALV-J strains isolated from layer flocks was investigated. A total of 205 nucleotides were deleted from the 3′ untranslated region of 89.5% (17/19) of the ALV-J layer isolates. Approximately 94.7% (16/17) of the layer isolates contained a complete E element of 146-149 residues. The U3 sequences of 84.2% (16/19) of the ALV-J layer isolates displayed less than 92.5% sequence homology to the ALV-J broiler isolates, although the transcriptional regulatory elements that are typical of avian retroviruses were highly conserved. Several unique nucleotide substitutions in the U3 region and the E element of most of the ALV-J layer isolates were detected. These results suggested that the E element and U3 region in the ALV-J layer isolates have evolved rapidly and were significantly different from those of the ALV-J broiler isolates. These findings will contribute to a better understanding of the pathogenic mechanism of layer tumor diseases induced by ALV-J.

中图分类号:

S852.659.3

高玉龙，贠炳岭，秦立廷，潘伟，王永强，高宏雷，祁小乐，王笑梅. 蛋鸡J亚群禽白血病病毒3′非编码区序列特征分析[J]. 畜牧兽医学报, doi: .

GAO Yu-long, YUN Bing-ling, QIN Li-ting, PAN Wei, WANG Yong-qiang, GAO Hong-lei, QI Xiao-le, WANG Xiao-mei. Molecular Characteristics of 3′ Untranslated Regions of Avian Leukosis Virus Subgroup J in Layer Flocks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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