5个家兔群体白细胞介素10基因外显子的遗传多样性分析

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (5): 723-728.doi:

5个家兔群体白细胞介素10基因外显子的遗传多样性分析

冒留留1,2，潘雨来3，王晓明1,2，万小颖1,2，李碧春1,2，吴信生1,2*

1.江苏省动物遗传繁育与分子设计重点实验室，扬州 225009； 2.扬州大学动物科学与技术学院，扬州 225009； 3.江苏省金陵种兔场，南京 211103

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 吴信生

Genetic Diversity of IL10 Gene Exons in 5 Rabbit Populations

MAO Liuliu1,2, PAN Yulai3, WANG Xiaoming1,2, WAN Xiaoying1,2,
LI Bichun1,2, WU Xinsheng1,2*

1.Jiangsu Key Laboratory of Animal Genetics, Breeding and Molecular Design, Yangzhou 225009, China； 2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China； 3.Jiangsu Jinling Rabbits Field, Nanjing 211103,China

Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
Contact: WU Xinsheng1

摘要/Abstract

摘要： 本研究旨在分析家兔IL10基因外显子的多态性，以期为进一步研究家兔IL10与疾病抗性的相关性提供理论依据。根据GenBank上收录的IL10基因序列设计5对特异性引物，采用PCRSSCP方法对海狸色獭兔、白色獭兔、皖系长毛兔、闽西南黑兔、九疑山兔这5个家兔群体IL10基因的5个外显子序列进行多态性分析。结果表明：在IL10基因外显子3上检测到4种等位基因，10种基因型，存在3个SNPs位点；在外显子4上检测到2种等位基因，3种基因型，存在1个SNP位点。而外显子1、2、5对于实验群体未发现有遗传多态性。在外显子3中，D等位基因只在闽西南黑兔和九疑山兔中检测到，除海狸色獭兔和闽西南黑兔外其余群体均处于哈代温伯格平衡，各群体不同基因型分布存在极显著差异（P<0.01）。在外显子4中，A1B1基因型在獭兔群体中没有检测到；除海狸色獭兔外其余各群体均处于哈代温伯格平衡，海狸色獭兔跟白兔獭兔不同基因型分布差异不显著（P>0.05），而其余群体彼此不同基因型分布存在显著性差异（P<0.05或P<0.01）。结果提示：5个家兔群体在IL10基因外显子3和4中存在遗传多态性，不同家兔群体在遗传基础上存在着一定的差异。

Abstract: This experiment was conducted to study the polymorphism of IL10 gene exons in rabbit and aimed to provide a theoretical foundation for further reseach on correlation between rabbit IL10 gene and disease resistance. Five exons of IL10 gene in 5 rabbit populations including Beaver Color Rex rabbit,White Rex rabbit,Wanline Angora rabbit,Jiuyishan rabbit and Minxinan Black rabbit were scanned by PCRSSCP method with five specific primers designed according to the IL10 sequence from GenBank. The results showed that 10 genotypes and 4 alleles were found in exon3 of IL10 gene which contained three SNPs, 3 genotypes and 2 alleles were found in exon4 which contained one SNP. There was no mutation found at exon1, exon2, exon5 of IL10 gene. In exon3, D allele was found only in Minxinan Black rabbit and Jiuyishan rabbit.The distribution of these genotypes in all populations was consistent with the HardyWeinberg equilibrium except Beaver Color Rex rabbit and Minxinan Black rabbit, which was very significantly different (P<0.01) among different populations. In exon4, A1B1 genotype was not found in Rex rabbit group.The distribution of these genotypes was significant different (P<0.05 or P<0.01) among different populations except between Beaver Color and White Rex rabbit (P>0.05). These results indicated that genetic diversity of rabbit IL10 gene was detected in exon3 and exon4.

冒留留;潘雨来;王晓明;万小颖;李碧春;吴信生;. 5个家兔群体白细胞介素10基因外显子的遗传多样性分析[J]. 畜牧兽医学报, 2012, 43(5): 723-728.

MAO Liuliu;PAN Yulai;WANG Xiaoming;WAN Xiaoying;LI Bichun;WU Xinsheng;. Genetic Diversity of IL10 Gene Exons in 5 Rabbit Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(5): 723-728.

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