Abstract: The experiment was conducted to study the enzyme activity and mRNA expression of malic enzyme (ME) induced by glucose and insulin in goose primary hepatocyte, and identify the role of malic enzyme in goose hepatic steatosis. In this study, partial sequence of ME gene was cloned using Sichuan White goose. The goose primary hepatocyte was cultured and hepatic steatosis was induced by means of adding different concentrations of glucose and insulin, malic enzyme activity and mRNA expression and the triglyeride (TG) content in cells tested were detected. The goose ME gene sequence had highest similarity with Gallus gallus. Compared with the control group, glucose and its synergy with insulin could significantly increase the TG content in a dose dependent manner, however, insulin alone failed to notably affect TG content; the enzyme activity and mRNA expression of ME gradually increased with glucose concentrations increasing, and 30 mmol·L-1 glucose could significantly increase those parameters（P＜0.05）; low contents of insulin（50 nmol·L-1）could largely elevate the enzyme activity and mRNA expression of ME(P＜0.05), which could be suppressed by 200 nmol·L-1 insulin. In addition, the synergy of glucose with lower content of insulin（50 nmol·L-1） could significantly increase the enzyme activity and gene expression of ME. In this study, the partial sequence of goose ME gene were cloned. Meanwhile, glucose and insulin could coordinately promote the mRNA expression and enzyme activity of ME in goose liver resulting in TG accumulation.