Abstract: The objective of this study was to study the transcription profiles of Etron2 gene during the different development stages. The parasites at different developmental stages were harvested and their total RNA were substracted by realtime PCR using Etactin gene as a reference for establishing standard curves to evaluate the copy number of Etron2 genes. All of quantitative analyses were reliable because the melting curve showed a single peak. Etron2 gene transcription reached a peak during the unsporulated oocysts stage, then merozoites, sporulated oocysts and the lowest during the sporozoites stage. It is likely that the transcription messages were stored during the unsporulated oocysts stage, and then the Etron2 protein can be translated and secreted into the rhoptry soon when the oocysts were sporulated, and it may play an important role in the process of invasion.