畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (4): 509-515.doi:

• 遗传繁育 • 上一篇    下一篇

FSHR基因第1外显子多态性及其与小梅山猪产仔数的关系

吴井生1,2,3,张跟喜1,2,戴国俊1,2,叶兰1,2,王金玉1,2*   

  1. 1.扬州大学动物科学与技术学院,扬州 225009; 2.江苏(扬州)规模化猪场高效健康养殖公共技术服务中心,扬州 225009; 3. 江苏农林职业技术学院,句容 212400
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-04-25 发布日期:2012-04-25
  • 通讯作者: 王金玉

Polymorphism of Exon1 of FSHR Gene and Its Relationship with Litter Size in Xiaomeishan Pigs

WU Jingsheng1, 2, 3,ZHANG Genxi1,2,DAI Guojun1,2,YE Lan1,2,WANG Jinyu1,2*   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2. Jiangsu (Yangzhou) Public Technical Service Centre for Efficient and Healthy Culture Technology in Largescale Swine Farms, Yangzhou 225009, China; 3. Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-04-25 Published:2012-04-25
  • Contact: WANG Jinyu

摘要: 本研究旨在检测猪FSHR基因第1外显子多态性,并分析其与产仔数之间的关系。采用PCRSSCP和直接测序方法检测基因多态性,利用最小二乘法分析其与母猪产仔数的关系,同时对多态位点的方差组分进行分析,并预测选择反应。研究结果表明:猪FSHR基因第1外显子区域内检测到3个SNPs(C70T、C74G、C81T),其中C74G导致氨基酸的改变,同时,筛选出3个单倍型和5种基因型;FSHR基因单倍型与基因型分布在小梅山猪、枫泾猪与大白猪群体间差异达到极显著水平(P<0.01);关联分析结果显示,2胎以上的小梅山母猪中,AA和CC基因型个体的TNB比BC基因型分别低0.64(P>0.05)和0.36头(P>0.05),所有胎次中,AA基因型个体的TNB和NBA比AC基因型分别低0.80(P<0.01)和0.67头(P<0.01)。对于FSHR基因第1外显子而言,小梅山猪TNB和NBA的遗传主要受到显性效应的影响。

Abstract: The present study was to detect the polymorphism of exon1 of folliclestimulating hormone receptor (FSHR) gene and investigate the relationship between FSHR gene and litter size in pigs. The polymorphism of exon1 of FSHR gene was investigated by PCRSSCP and direct sequencing methods and the effect of exon1 of FSHR gene on litter size in Xiaomeishan pigs was analyzed using the least square analysis, simultaneously the variance components were analyzed and the selective reaction was predicted. The results showed that three SNPs (C70T, C74G and C81T) were detected in exon1 of FSHR gene, of which C74G caused amino acid change. Simultaneously three haplotypes and five genotypes were screened out. The difference of FSHR gene haplotypes and genotypes distribution among Xiaomeishan pigs, Fengjing pigs and Large White pigs reached extremely significant level (P<0.01). According to the result of correlation analysis, in Xiaomeishan pigs, the TNB of the sows with genotype AA and AC after the second parity were 0.64 (P>0.05) and 0.36 (P>0.05) piglets less than those with genotype BC, and the TNB and NBA of the sows with genotype AA in all parities were 0.80 (P<0.01) and 0.67 (P<0.01) piglets less than those with genotype AC. The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic dominant effect in exon1 of FSHR gene.