Abstract: Limousin bulls and Charolais bulls were used in the present study to examine the influence of different seasons on semen quality, frozen semen productivity, physiological response, blood biochemical parameters and seminal plasma biochemical parameters. Results showed that: (1)The sperm motility, frozen sperm motility, percentage of motile sperm and percentage of intact acrosome were significantly lower, and the rate of abnormal sperm is significantly higher in summer than that of other seasons(Ｐ<0.05), while sperm concentration was at the lowest level in autumn.(2)The quantity of frozen semen production in summer was at the lowest level, only 7 388 count each month. (3)The mean concentrations of blood serum LH(48.12 ng/L), testosterone(4.15 ng/L), T3(1.11 ng/L) and cortisol(4.89 ng/L) were lower in summer than that of other seasons(Ｐ<0.01). (4)Compared with other seasons, the mean levels of blood serum sodium, potassium, calcium and magnesium decreased significantly in summer(Ｐ<0.01).(5) The concentration of seminal plasma testosterone of beef breeding bull was lower in summer than that of spring, autumn and winter significantly(Ｐ<0.05). (6) the mean concentrations of seminal plasma sodium(83.12 mmol/L), potassium(18.77 mmol/L) and calcium(4.63 mmol/L) were at the lowest level in summer compared with that of spring, autumn and winter; the concentration of magnesium in seminal plasma had the significant drop trend from spring to autumn, but the mean concentrations of seminal plasma phosphorus (4.65 mmol/L) in summer was on the highest level and significantly higher than that of other seasons(Ｐ<0.01). (7) In summer, the mean concentration of seminal plasma ALP(1 784.3 U/L) was higher than that of spring and autumn(Ｐ<0.05), but lower than that of winter (Ｐ<0.01).(8) Seminal plasma testosterone was found to be significantly correlated with the parameters of seminal quality(Ｐ<0.05). The serum biochemical parameters were significantly correlated with seminal plasma biochemical parameters(Ｐ<0.05). The respiratory rate and rectal temperature were found to be significantly correlated with the parameters of seminal quality and serum，but there was no significant correlation with seminal plasma biochemical parameters(Ｐ<0.05).