猪氨基酸转运载体b0,+AT-2的分子克隆及其序列分析

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (4): 595-601.doi:

猪氨基酸转运载体b^0,+AT-2的分子克隆及其序列分析

职爱民^1,2，冯定远¹*，周响艳³，黄志毅¹，邹仕庚¹，王修启¹，左建军¹，叶慧¹，王小兰¹，刘准¹

1. 华南农业大学动物科学学院，广州 510642； 2. 河南农业科学院河南省动物免疫重点实验室，郑州 450001； 3. 东莞泛亚太生物技术有限公司，东莞 523808

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 冯定远

Molecular Cloning and Sequence Analysis of the Amino Acid Transporter ^b0,+AT-2 of Pigs

ZHI Ai-min^1,2, FENG Ding-yuan¹*, ZHOU Xiang-yan³, HUANG Zhi-yi¹, ZOU Shi-geng¹,WANG Xiu-qi¹, ZUO Jian-jun¹, YE Hui¹, WANG Xiao-lan¹, LIU Zhun¹

1.College of Animal Science, South China Agricultural University, Guangzhou 510642, China；2. Henan Provincial Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450001, China; 3.ASIAPAC (Dongguan) Biotechnology Co,Ltd, Dongguan 523808, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-04-25 Published:2009-04-25

摘要/Abstract

摘要： 本研究克隆了^b0,+AT-2的全长cDNA序列。基于NCBI发布的人和鼠b^0,+AT序列，应用生物学软件进行序列比对后在同源区设计引物，获取cDNA片段；再利用RACE技术克隆了其全长cDNA序列。生物信息学分析显示：猪b^0,+AT-2的全长1 488 bp，包括90 bp的3′非翻译区（UTR）和126 bp的5′UTR；编码区（CDS）编码423个氨基酸残基，分别和人、鼠b^0,+AT以及猪b^0,+AT-1有75.1%、71.9%和86.6%的同源性。

Abstract: The cloning, sequencing of porcine b^0,+AT transcript variant 2 was described in this study. Based on the sequences of human and mouse b^0,+AT published on the NCBI, we identified a partial cDNA using the primers designed after bioinformatics analysis. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine b^0,+AT-2 was sequenced. The porcine b^0,+AT-2 cDNA was 1 488 bp long, including 90 bp 3′UTR and 126 bp 5′UTR, and CDS encoded 423 amino acids. The b^0,+AT-2 protein sequence was found to be 75.1%，71.9% and 86.6% identities with human b^0,+AT, mouse b^0,+AT and swine b^0,+AT-1.

职爱民;冯定远;周响艳;黄志毅;邹仕庚;王修启;左建军;叶慧;王小兰;刘准. 猪氨基酸转运载体b^0,+AT-2的分子克隆及其序列分析[J]. 畜牧兽医学报, 2009, 40(4): 595-601.

ZHI Ai-min;FENG Ding-yuan;ZHOU Xiang-yan;HUANG Zhi-yi;ZOU Shi-geng;WANG Xiu-qi;ZUO Jian-jun;YE Hui;WANG Xiao-lan;LIU Zhun . Molecular Cloning and Sequence Analysis of the Amino Acid Transporter ^b0,+AT-2 of Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(4): 595-601.

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