秦川牛LXRα基因第二外显子多态性及其与部分胴体、肉用性状关联性研究

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (5): 531-535.doi:

秦川牛LXRα基因第二外显子多态性及其与部分胴体、肉用性状关联性研究

黄磊¹ ，昝林森^1,2*，王洪宝^1,2，刘洪瑜¹

1. 西北农林科技大学动物科技学院, 杨凌 712100；2. 国家肉牛改良中心，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-25 发布日期:2010-05-25
通讯作者: 昝林森

Study on SNP of LXRα Gene Exon2 and Its Association with Some Meat Traits in Qinchuan Cattle

HUANG Lei¹, ZAN Lin-sen^1,2*, WANG Hong-bao^1,2, LIU Hong-yu¹

1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100,China; 2. National Beef Cattle Improvement Centre in China,Yangling 712100, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25

摘要/Abstract

摘要： 旨在分析秦川牛肝X受体基因(LXRα)第二外显子的遗传变异与部分酮体、肉用性能指标的相关性。随机选择相同饲养条件下的497头18~20月龄秦川牛阉牛，采用PCR-SSCP技术进行了LXRα基因部分区段遗传变异检测，运用SPSS程序中的GLM模型分析所检测到的遗传变异与秦川牛部分肉用性能指标的关联性。结果，找到了LXRα基因DNA序列中的一个突变位点T1530C(NC_007313)。对该遗传变异结果进行分型并与114头秦川牛的肉用性状进行关联分析，结果表明，该位点的多态性与胴体长、大理石花纹评分和背膘厚之间存在显著相关（P<0.05）；BB和AB基因型个体胴体长显著高于AA基因型个体（P<0.05），BB基因型个体大理石等级和背膘厚显著高于AA基因型个体（P<0.05），与AB基因型个体差异不显著（P>0.05）。试验结果表明该SNP位点的BB基因型为优势基因型，与胴体长、背膘厚、大理石花纹等肉用性状有相关性，提示LXRα基因能够作为分子标记辅助选择的候选基因。

Abstract: The aim of this study was to analyze the association between hereditary variation of bovine LXRα exon2 and some meat traits. Four hundred and ninty-seven of 18-20 months old Qinchuan steers under identical feeding conditions were selected randomly and variation of LXRα gene was analyzed by PCR-SSCP technique. The association between SNP locus and some meat traits was analyzed using the general linear model (GLM) in SPSS program. One polymorphic site T1530C was found (NC_007313). Genotypic variation had a significant effect on backfat thickness, carcass length and marbling score (P<0.05).The carcass length of individuals with BB and AB genotypes was significantly different from that of individuals with AA genotype (P<0.05). The marbling score and backfat thickness of individuals with BB genotype were significantly different from that of individuals with AA genotype (P<0.05). The results indicated that BB genotype was favorable genotype at this SNP locus and was highly associated with seven meat traits, which suggested that LXRα gene could be studied as a candidate gene for molecular marker assisted selection(MAS).

黄磊;昝林森;王洪宝;刘洪瑜. 秦川牛LXRα基因第二外显子多态性及其与部分胴体、肉用性状关联性研究[J]. 畜牧兽医学报, 2010, 41(5): 531-535.

HUANG Lei;ZAN Lin-sen;WANG Hong-bao;LIU Hong-yu. Study on SNP of LXRα Gene Exon2 and Its Association with Some Meat Traits in Qinchuan Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(5): 531-535.

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