Abstract: The HA gene from subtype H9N2 AIV was directionally inserted into the vector pP12LS, resulting in transfer vector pP12LSH9A. Then the pP12LSH9A was used to transfect the chicken embryo fibroblast cells, which were preinfected with strain 282E4 or LP of FPV, respectively. After serial blue plaque screening, we obtained two purified rFPVs: rFPV₂₈₂-12LSH9A and rFPV_LP-12LSH9A. SPF and antibody positive commercial chickens were immunized with the two rFPVs to investigate their protective efficacies. Chickens immunized with homologous AIV inactivated vaccine or widetype FPV served as controls.The protective efficacies of rFPV₂₈₂-12LSH9A and rFPV_LP-12LSH9A in SPF chickens were both 100%，those ones in commercial chickens were 88% and 92%, respectively. The results revealed that the rFPV₂₈₂-12LSH9A and rFPV_LP-12LSH9A are two effective candidate vaccines against H9 subtype AIV even in the presence of maternal antibodies against H9 subtype AIV.