Abstract: The Jinhua pigs (40), Pietrain pigs (30) and the second generation of crossbred of Jinhua and Pietrain (126) were used in the study, their genotype of SNPs at the position 187 of exon 44 and at the position 37 and 179 of intron 37 for CACNA1S were investigated by PCR-RFLP. The association analysis of the SNPs genotype with the meat quality traits was also conducted in the study, results showed that the genotype AA of Eco 72 I had significant effects on M longissimus dorsi area and meat lightness (L) (P<0.01), the genotype BB of Eco 72 I had significant effects on lean meat weight (P<0.01) and pH value for ham (0.01＜P＜0.05). The site Xba I had significant effect on backfat thickness at the thorax and waist joint position (0.01<P<0.05), but no significant difference was found among the genotype AA, AB and BB. The site Cfr 42 I showed polymorphisms only in Jinhua and Pietrain pigs and with the only genotype BB in the second generation of crossbred of Jinhua and Pietrain.