Abstract: Porcine surfactant protein A (SP-A) is the most in content and the first found in porcine surfactant protein which has the function of regulating inflammation and immune. The complete DNA sequence of porcine SP-A was attained by designing six primers during PCR cloning in this study. The length of the complete DNA sequence of porcine SP-A was 4 037 bp (gi: DQ985806) including the promoter regions , a 747 bp open reading frame coding 248 amino acid(aa). The molecular weight and the isoelectric point of porcine surfactant protein A was 26.37 ku and 5.20, respectively. It’s predicted the 1-20 aa was signal peptide sequence, and the 1-20 aa，120-135 aa, 145-160 aa was hydrophobicity domain; porcine surfactant protein A was in the surface of membrane by transmembrane helices analysis. Meanwhile, as human SP-A, the porcine SP-A has conservative collagen-like region, neck region, and carbohydrate recognition domain . The present results would contribute to study on the association between SP-A and porcine respiration disease .