Abstract: Based on the mRNA sequence of human Suv39h2 gene, a porcine fragment of its cDNA sequence was obtained using in silico cloning method. By RT-PCR method, a porcine Suv39h2 cDNA fragment of 1 291 bp was amplified from muscle tissue and inserted into pGEX-KG plasmid expressing glutathione Stransferase (GST) fusion protein. After confirmation by the sequencing and restriction enzyme analysis, the recombinant plasimids were transformed into E. coli BL21 competent cells. The optimal IPTG induction conditions were determined as follows: induction time was 4 h and IPTG concentration was 0.7 mmol·L^-1. The fusion protein was insoluble. Using Western blotting, we found a band with molecular weight of 66 kD, which indicated that porcine Suv39h2 was expressed in E. coli. This work laid a foundation for further work on functional study.