Myostatin基因5′调控区的多态与猪生长性能的关系

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (5): 617-621.doi:

Myostatin基因5′调控区的多态与猪生长性能的关系

吴俊红，武艳群，赵晓枫，吴旧生，徐宁迎*

浙江大学华家池校区动物科学学院，杭州 310029

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25
通讯作者: 徐宁迎

Relationship between Polymorphism of 5′Regulatory Region of Porcine MSTN and Growth Performance

WU Jun-hong, WU Yan-qun, ZHAO Xiao-feng, WU Jiu-sheng，XU Ning-ying*

College of Animal Science，Zhejiang University，Hangzhou 310029, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要/Abstract

摘要： 采用PCR-RFLP技术对45头金皮(金华猪×皮特兰猪)F₂代猪Myostatin基因5′调控区的多态性进行检测，根据酶切结果将其分为AA、TT和AT 3种基因型，结果显示A等位基因频率为0.467，T等位基因频率为0.533。对这些个体进行生长和屠宰性能测定以及对背最长肌进行免疫组化分析，进而采用SPSS程序分析Myostatin不同基因型对金皮F2代猪生长性状、肌纤维组织学特性及胴体性状的影响。结果表明：Myostatin基因对金皮F₂代猪肌纤维直径、MHC I型肌纤维比例、肩部背膘厚、眼肌肉色亮度及出生至4月龄平均日增质量的影响均达到显著水平(P<0.05)。

Abstract: The polymorphism of the 5′regulatory region of Myostatin gene was detected using PCR-RFLP method in 45 Jinpi F2 pigs.The results showed that there were 3 genotypes (AA, TT and AT) and their frequencies were 0.244, 0.311 and 0.444, respectively. Growth, carcass traits and Longissimus dorsi muscles analyzed using immunohistochemical were recorded. The results showed that the DraI polymorphism of MSTN gene had significant effect(P<0.05) on the muscle fiber diameter, the percentage of MHC type I fiber, the backfat thickness (shoulder)，the average daily gain from birth to 4 months old and the eye muscle lightness.

吴俊红;武艳群;赵晓枫;吴旧生;徐宁迎. Myostatin基因5′调控区的多态与猪生长性能的关系[J]. 畜牧兽医学报, 2009, 40(5): 617-621.

WU Jun-hong;WU Yan-qun;ZHAO Xiao-feng;WU Jiu-sheng;XU Ning-ying. Relationship between Polymorphism of 5′Regulatory Region of Porcine MSTN and Growth Performance[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(5): 617-621.

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