畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (5): 664-669.doi:

• 遗传繁育 • 上一篇    下一篇

北京鸭H-FABP多态与屠体性状相关分析

朱祥云1,2,4,杨晓刚1,3,4,王杰1,刘小林4,侯水生1*,黄苇1,俞俊英1,赵玲1   

  1. 1.中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室,北京 100193;2.中国水产科学研究院长江水产研究所,荆州 434000 ; 3.内蒙古呼伦贝尔学院,呼伦贝尔 021008;4. 西北农林科技大学动物科技学院,杨凌 712100
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 侯水生

Analysis on the Association of Polymorphism of H-FABP Gene with Carcass Traits of Peking Duck

ZHU Xiang-yun1,2,4, YANG Xiao-gang1,3,4, WANG Jie1, LIU Xiao-lin4,
HOU Shui-sheng1*, HUANG Wei1, YU Jun-ying1, ZHAO Ling1
  

  1. 1.State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193,China; 2.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000,China; 3. HuLunbeier University, HuLunbeier 021008,China; 4.College of Animal Science and Technology, Northwest A & F University, Yangling 712100,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要: 应用聚合酶链式反应-单链构象多态性(PCR-SSCP)的方法,以心脏-脂肪酸结合蛋白基因(H-FABP)为侯选基因,对6周龄北京鸭的3个群体(区分公母)共300个个体进行SNP分析。结果发现HFABP第3内含子以本试验采用的引物碱基为第一碱基的第156碱基处发生1个C/G突变,此突变对Z4群体胴体质量、皮脂质量、皮脂率和全净膛质量有显著影响,并且对Z4(公)群体中胸肌质量、腿肌质量以及骨架质量有显著影响,因此可以将心脏-脂肪酸结合蛋白基因作为影响北京鸭Z4群体屠体性状的侯选基因。

Abstract: In this paper, 300 Peking ducks were used to analyze H-FABP gene polymorphism by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). The results showed that the polymorphism of the 3rd intron was caused by C/G mutation at 156 bp. In three populations(males and females), only the Z4 population resulted in genotypes which showed a significant difference in carcass weight, abdominal fat weight and eviscerated weight (P<0.05),the Z4(male) population resulted in genotypes which showed a significant difference in breast weight, leg weight,and skeleton weight. It implied that H-FABP could have a major effect on carcass performance or could be linked to genes that significantly affect carcass performance in Z4 population of Peking duck.