Abstract: Genetic polymorphism of goose Pit-1 gene fragment was analyzed in 6 goose populations. The primer was designed according to chicken Pit-1 mRNA and genomic sequence. Two insertion/deletion mutations were found in the sequence of Pit-1 gene, and 3 genotypes AA, AB and BB were detected in 6 goose populations. Compared with AA genotype, there were 3 and 13 bp insertions in BB genotype after position 132 and 145 bp，respectively. All the populations were in Hardy-Weinberg equilibrium at this polymorphic site (P>0.05). The genotype distribution in Landosie was significantly different with that in other goose populations (P<0.01). The genotype distribution in Zi goose and Shitou goose were significantly different with that in other populations（P<0.05）. Allele A was the dominant allele in Shitou and Landoise goose populations, while allele B was the dominant allele in Wanxi White goose, Siji goose and Zhedong White goose populations.