Abstract: OBR (obese receptor) gene plays an important role in leptin functioning signal transduction. The polymorphism of the OBR-exon 20 was detected, and a novel SNP was found by the technique of single strand conformation polymorphism (SSCP) and finally confirmed by sequencing. The frequencies of different genotypes in cross combinations of local chickens were calculated and the relationship between three genotypes and fattiness traits of these chickens were analyzed based on liner model. The results showed that the novel SNP was at 860th locus (T/C) in OBR-exon 20, genotype frequencies of AA, AB, BB were 0.641, 0.292 and 0.067, respectively, in trial group. The effect of various genotypes on abdominal fat percentage, subcutaneous fat thickness, intramuscular fat width and intramuscular fat percentage were not significant (P＞0.05). It could be a reference for future research on polymorphism of OBR and supply more information for gene mapping.