Abstract: To investigate the pharmacokinetics of Compound HuangBo Granule in mice, the effective component-berberine was detected as a representative index.Pharmacokinetics of orally administration of Compound HuangBo Granule at a dose of 25 g/kg (10.95 mg/kg berberine) were investigated . Serum concentration of berberine were measured using HPLC and concentration-time data were analyzed with pharmaceutical kinetics software. The results showed that the serum profiles of the drug's effective component-berberine were best described by a two compartment open model, the pharmacokinetics equation is C=17.8e^{-0.859 81t}+14.51 e^{-0.636 1t}.The main parameters were as follows: t_1/2α(0.81±0.07) h, t_1/2β(5.25±2.96) h, t_1/2Ka(0.76±0.05) h, AUC_0→∞(3.389 3±0.4３7 4) mg·L^-1·h, CL/F (3.281 8±0.446 3) L/h, V_^d/F(13.65±22.38) L/kg, T_peak(1.617 19±0.056 5０) h, C_max(1.149 98±0.056 04) μg/mＬ.