Abstract: For investigating the effects of Shenpang acupoint-stimulation on reproductive endocrine, the changes of estrogen receptors immunoreactive (ER-IR) neurons after Shenpang acupoint-stimulation were studied by using the technique of immunohistochemistry SP. The ER-IR positive productions were detected in most nuclei of thalamus. In acupuncture group, a great number of ER-IR positive productions, with clear dendrite, existed in the nucleus paraventricularis thalami, nucleus ventralis lateralis thalami, nucleus ventralis medialis thalami, nucleus ventralis principalis thalami, nucleus centromedianus thalami, nucleus reticularis thalami, nucleus periventricularis thalami, and were stained strongly. In addition, the ER-IR positive production mainly located in plasma, nucleus and neurite, also existed in the cytoplasmic membrane. In contrast, a few of neurons existed in the above nuclei in control group, and the neurons were stained weakly. The expression of estrogen receptors in the above nuclei increased after the Shenpang acupoint-stimulation.