畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (3): 309-312.doi:
• 研究简报 • 上一篇
方晓敏;赵晓枫;郭晓令;徐宁迎
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FANG Xiao-min;ZHAO Xiao-feng;GUO Xiao-ling; XU Ning-ying
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摘要： CACNA1S是钙离子通道α1亚单位的编码基因，该基因突变会导致人低钾性周期性麻痹和猪恶性高温综合征。根据人CACNA1S基因3′端非翻译区序列设计引物， 对猪基因组DNA进行PCR扩增、测序， 与人相应序列作同源性比较；然后采用IMpRH panel将CACNA1S基因定位于猪10p11-12。
Abstract: CACNA1S gene encodes the α1 subunit of Calcium channel. The mutation of CACNA1S gene can lead to hypokalemic periodic paralysis in human and Malignant hyperthermia in porcine. Primers were designed according to the published human CACNA1S sequences for amplification of porcine CACNA1S gene. PCR products were sequenced and compared with that of human. CACNA1S gene was located at 10p11-12 in porcine by IMpRH panel (INRA-University of Minnesota porcine Radiation Hybrid panel).
方晓敏;赵晓枫;郭晓令;徐宁迎. 应用辐射杂种细胞系对猪CACNA1S基因的定位研究[J]. 畜牧兽医学报, 2006, 37(3): 309-312.
FANG Xiao-min;ZHAO Xiao-feng;GUO Xiao-ling;XU Ning-ying. Mapping CACNA1S Gene in Porcine by Radiation Hybrid Panel[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(3): 309-312.
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