Abstract: The aim of this study was to clone the cDNA of PGC-1β and PRC gene from the heart of porcine by RT-PCR approach and to analyze its sequence and expression profile. According to the PGC-1β and PRC mRNA sequences of human which were the homologous sequences of pig searched in the EST library, the partial PGC-1β and PRC cDNA sequence of pigs were cloned by RT-PCR, and their expression profile were detected in heart, muscle, spleen, liver, lung, kidney, small intestine and adipose tissue by using RT-PCR and Western blot. The result showed that the PGC-1β and PRC were 1 251 and 1 254 bp in length, and encoding 415 and 417 amino acids(GenBank accession number：FJ377680 and FJ479491), respectively. Bioinformatic analysis found that the homology of nucleotide sequence and amino acid were about 80% compared with the counterpart sequences of other species, and containing TPPTTPP and DHDY conserved sequences, as well as the RNA recognition motif(RRM)conserved domains. PGC-1β was widely expressed in 8 tissues,while PRC was highly expressed only in heart, liver, skeletal muscle and kidney,but not detected in spleen and small intestine. The result suggested that the differention of expression level of the PGC-1β and PRC may be related to their physiological functions in different tissues.The results will be helpful for elucidating the physiological function and regulatory mechanisms of PGC-1 family.