西藏日土藏山羊ISSR标记遗传多态性研究

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (9): 1208-1212.doi:

西藏日土藏山羊ISSR标记遗传多态性研究

王永¹*，王杰¹，许期树¹，字向东¹，欧阳熙¹，刘鲁蜀¹，小益西²

1. 西南民族大学青藏高原动物遗传资源保护与利用四川省重点实验室，成都 610041；2. 西藏自治区农牧科学院，拉萨 850000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
通讯作者: 王永

Analysis of Genetic Diversity in Ritu Tibetan Goats by ISSR

WANG Yong¹*, WANG Jie¹, XU Qi-shu¹, ZI Xiang-dong¹, OUYANG Xi¹, LIU Lu-shu¹, XIAO Yi-xi²

1. Sichuan Provincial KeyLaboratory of Protection and Utilization of Animal Genetic Resources in QinghaiTibet Plateau, Southwest University for Nationalities, Chengdu 610041, China; 2. Academy of Agriculture and Animal Husbandry of Tibet Autonomous Region, Lasa 850000， China

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20

摘要/Abstract

摘要： 应用ISSR标记分析西藏日土藏山羊的遗传多样性，为其保护和开发利用提供基础资料。从93条ISSR引物中筛选出10条对107只藏山羊进行分析，数据分析利用Excel和SPSS软件完成。结果表明，所筛选出的10条引物特异性较好且多态性较高，共扩增出112条清晰的条带，平均每条引物扩增出11.2条带，其中多态性条带75个，群体多态位点百分率(P)为66.96%，扩增片段大小为219～2 534 bp，群内个体间遗传相似性指数(s)为0.460 0～0.740 5，平均0.564 7，个体间平均遗传差异(D)为 0.435 3。根据多态性位点在样本中出现的几率计算出多态性条带的基因频率（f），频率值的范围为0.045 5～1.000 0。西藏日土藏山羊的遗传多态性较丰富，个体间存在差异，但又具有较好的同质性。

Abstract: Inter simple sequence repeat(ISSR)was used to analyze the genetic diversity of Tibetan goats population in Ritu county. Ten primers were selected from 93 ISSR primers and then used to detect the genetic diversity in 107 Tibetan goats. The obtained data were analyzed by using Excel 2003 and SPSS11.0 softwares. Ten out of 93 ISSR primers produced 112 DNA bands, among them 75 bands were polymorphic markers and the size of amplified fragment ranged from 219 to 2 534 bp. It indicated that the selected primers were specific and could produce enough polymorphic bands. The proportions of polymorphic loci(P)in Ritu Tibetan goats was 66.96%. The genetic similarity index(s)within the detected population ranged from 0.460 0 to 0.740 5(with average of 0.564 7). The averaged individual genetic difference(D)was 0.435 3. The gene frequency(f)ranged from 0.045 5 to 1.000 0. These results of ISSR analysis indicated that the Ritu Tibetan goats population presents high level of genetic diversity and genetic variations existed among individuals.

王永;王杰;许期树;字向东;欧阳熙;刘鲁蜀;小益西. 西藏日土藏山羊ISSR标记遗传多态性研究[J]. 畜牧兽医学报, 2010, 41(9): 1208-1212.

WANG Yong;WANG Jie;XU Qi-shu;ZI Xiang-dong;OUYANG Xi;LIU Lu-shu;XIAO Yi-xi. Analysis of Genetic Diversity in Ritu Tibetan Goats by ISSR[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(9): 1208-1212.

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