Abstract: 120 1-day-old parental male Arbor Acre (AA) broiler and 120 1-day-old parental male Lingnan yellow broiler were randomly divided into 4 replicates, respectively. Regional expression of Peptide transporter 1 (PepT1) mRNA in different intestinal tract segments of Lingnan yellow broiler on 30 d and ontogeny of PepT1 mRNA in duodenum and jejunum of AA broiler and yellow broiler were determined by relative quantitative RT-PCR. The results showed that: (1) Regional expression of PepT1 mRNA in duodenum, jejunum, ileum and colorectum was declined gradually. The abundance of PepT1 mRNA in duodenum was higher than in colorectum (P<0.05)； (2)Ontogeny patterns of PepT1 mRNA in duodenum and jejunum were consistent between AA broiler and Lingnan yellow broiler. The abundance of PepT1 mRNA in two strains was the highest on day 16, decreased from day 16 to day 44 and slightly increased again on day 58. While compared two breeds directly, the abundance of PepT1 mRNA in AA broiler was not significant difference than that in Lingnan yellow broiler from day 2 to day 58(P>0.05). These results indicated that： (1) Regional expression of PepT1 mRNA in intestine of Lingnan yellow broiler was declined gradually along the downward intestine from duodenum, jejunum, and ileum to colorectum. The abundance of PepT1 mRNA in duodenum was higher than in colorectum (P<0.05)；（2） Ontogeny of PepT1 mRNA in two breeds had the same pattern in duodenum and jejunum, and there was no difference at same development stage between AA broiler and Lingnan yellow broiler, indicating that PeptT1 mRNA expression could be regulated by developmental stage but not breeds.