Abstract: The residue was extracted by hot methanol from Oxytropis glacialis powder, acidified with 1mol/L HCl and filtrated to get acid liquid. The acid liquid was extracted with chloroform to remove impurity. Then, its pH was adjusted to 9-10 with NaOH. The solution was extracted respectively with chloroform，ethyl acetate and n-butanol. The most alkaloids were maximum polarity in the butanol phase, with TLC checking on alkaloid，there were 10 alkaloids in chloroform phase, 7 alkaloids in ethyl acetate and 5 alkaloids in butanol phase. Applied the crude alkaloid extracted with butanol to a silica gel column, eluted with different proportion of ethyl acetate and methanol, collected 30-50 mL as one sample, monitored by TLC, incorporated the same fraction. The Ehrlich’s colorable fraction were sublimated and white needles crystal were obtained. The crystal was detected by TLC, which appeared the same dot shape, color and Rf as the standard swainsonine. The results preliminary determined that crystal was swainsonine. The chemical structures were identified as swainsonine by means of MP and IR, MS, ¹HNMR, ¹³CNMR.