Abstract: 1-day-old chicks fed with commercial probiotics or home-made probiotics 3 days, the experimental chicks were taken to collect thymus, spleen, bursa of fabricius, tear, trachea fluid, bile and intestinal fluid to detect the dynamic changes of immune organs index and the relative content of immunoglobulin(Ig)A,IgG,IgM by application of indirect enzyme-linked immunoassay(ELISA) test after administration probiotics 1,4,7,10,18 days. The results were as follows: immune organs index of chicks after administration probiotics 7 days was enhanced compared with those of without probiotics, the relative content of IgA,IgG,IgM in local humor of chicks after administration commerical probiotics 7~10 days was enhanced compared with those of without probiotics, the content of IgA,IgG,IgM in local humor of chicks after administration home-made probiotics 4~7 days was enhanced compared with those of without probiotics.The results showed that probiotics have effect on mucosal immune and immune system of chicks.