牛TG基因启动子区遗传变异与肉质和胴体性状的相关性研究

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (3): 315-319.doi:

牛TG基因启动子区遗传变异与肉质和胴体性状的相关性研究

张路培¹，任红艳¹，甘乾福²，李俊雅¹*，李恒德¹，许尚忠^{1, 2}*，高雪¹，陈金宝¹

1.中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室，北京 100193；2. 西北农林科技大学动物科技学院，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-24 发布日期:2009-03-24
通讯作者: 许尚忠;李俊雅

Genetic Variations in Promoter Region of TG Gene and Its Association with Carcass and Meat Quality Traits in Cattle

ZHANG Lu-pei¹, REN Hong-yan¹, GAN Qian-fu², LI Jun-ya¹*, LI Heng-de¹,
XU Shang-zhong^1，2*,GAO Xue¹, CHEN Jin-bao¹

1.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry Agriculture,Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193， China；2. College of Animal Science and Technology, Northwest A & F University, Yangling 712100， China

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-24 Published:2009-03-24

摘要/Abstract

摘要： 以8个肉牛品种为试验材料，扩增TG基因启动子区，测序后发现G82A和C422T 2个突变位点。利用SAS软件采用最小二乘法拟合线性模型将2个位点的不同基因型与牛胴体组成和肉质性状进行关联分析。结果发现，C422T位点与先前报道的大理石花纹等级无相关性，与其它胴体组成和肉质性状也未发现相关性。位于C422T位点上游的G82A位点与宰前活体质量和眼肌面积显著相关(P<0.05)，GG为有利基因型，G等位基因为有利等位基因。因此，G82A可能是影响牛胴体性状的分子标记。

Abstract: In this experiment, promoter region of TG gene from eight breeds was amplified and sequenced. Two SNPs were identified at position 82 and 422 of TG gene (X05380). The effects of these polymorphisms of TG gene on carcass and meat quality traits were analyzed. The results showed that C422T polymorphism had no association with marbling score and any other carcass and meat quality traits. G82A polymorphism significantly associated with live weight and loin muscle area(LMA)(P<0.05). Individuals with genotype GG and GA had significantly larger live weight and LMA than those with AA genotype (P<0.05). In a word, G82A SNP might be a good DNA marker to be used in MAS for carcass traits.

张路培;任红艳;甘乾福;李俊雅;李恒德;许尚忠;;高雪;陈金宝. 牛TG基因启动子区遗传变异与肉质和胴体性状的相关性研究[J]. 畜牧兽医学报, 2009, 40(3): 315-319.

ZHANG Lu-pei;REN Hong-yan;GAN Qian-fu;LI Jun-ya;LI Heng-de;XU Shang-zhong;GAO Xue;CHEN Jin-bao. Genetic Variations in Promoter Region of TG Gene and Its Association with Carcass and Meat Quality Traits in Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(3): 315-319.

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