Abstract: Agouti gene plays an important role in the pigment synthesis of domestic animals. A SNP-T128 del was found by sequencing. The genetic diversity of T128 del in ten Chinese indigenous goat breeds was analyzed, as well as the relationship between the genotypes and the coat color by PCR-RFLP. Two alleles of A (non-del) and B (del), three genotypes of AA, AB and BB were detected. The genotype AA was superior in brown coat color goat breeds (77.41%); genotype AB existed mainly in white coat color goat breeds (76.14%) and the goat breeds with black coat color had BB genotype mostly (87.92%), so it was deduced that the T128 del might has the effect on the pigment synthesis of goat. The expected heterozygosity, the gene flow and the mean coefficient of gene differentiation of T128 del among ten goat breeds were 0.471 8, 0.319 0 and 0.439 3, respectively. The results indicated that most goat breeds investigated here were poor in genetic diversity at T128 del.