Abstract: To analyze mitochondrial DNA Dloop region sequences of Tibet Minipigs，and to explore their cytoplasmic DNA markers used to identify them and the phylogenetic relationship between Tibet Minipigs and other local pig breeds in China, mtDNA Dloop region in 102 Tibet Minipigs, 16 Bama miniature pigs and 17 Guizhou Xiang pigs were amplified and sequenced, followed by comparing with the data of other Chinese domestic pigs. The result showed that the Dloop region of Tibet Minipigs was divided into three areas. The tandem repeat sequence which had 1529 10 bp repeat motives and located in the middle of mtDNA Dloop region consisting of type A and B. 340 bp sequence at 3′ end of the Dloop which was very conservative exhibited particularly high homology with the same region of other domestic pigs. 704 bp sequence at 5′ end of the Dloop had 22 polymorphic sites. 22 polymorphic sites deduced 25 haplotypes, among which the percentage of two major haplotypes reached 344% and 366%, respectively. Based on three transform sites (305, 500, 691), Tibet Minipigs were divided into two groups, nearly corresponding to type A and B of tandem repeat sequence. Compared with Tibet Minipigs, Bama miniature pigs and Guizhou Xiang pigs had four and two haplotypes, respectively, and had type A of tandem repeat sequence. It is inferred that Tibet Minipigs originated from two maternal ancestors and were close relative relationship with local pigs in southwest China. The repeating motif in combination with the variable sites at the 5′end of mtDNA Dloop region can be uesd as the genetic marker to identify Tibet Minipigs.