GHRL及其受体GHSR基因多态性与鸭生长及屠体性状的关联性

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (1): 18-24.doi:

GHRL及其受体GHSR基因多态性与鸭生长及屠体性状的关联性

方梅霞^1，2，李莹²，徐海平²，谢亮²，廖新俤²，梁敏³，聂庆华²，张细权²*

1 暨南大学医学院实验动物管理中心，广州510632；2 华南农业大学动物科学学院，广州510642；3 广东省佛山市三水联科鸭场，佛山528000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-25 发布日期:2011-01-25
通讯作者: 张细权

Associations of Ghrelin (GHRL) and Its Receptor (GHSR) Genes Polymorphismswith Duck Growth and Carcass Traits

FANG Mei-xia^{1, 2}, LI Ying², XU Hai-ping², XIE Liang², LIAO Xin-di², LIANG Min³,
NIE Qing-hua², ZHANG Xi-quan²*

1.Laboratory Animal Management Center, School of Medicine, Jinan University, Guangzhou 510632, China; 2.College of Animal Science, South China Agricultural University， Guangzhou 510642, China;3. Sanshui Lianke Duck Farm, Foshan 528000, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

摘要/Abstract

摘要： 本研究旨在分析GHRL和GHSR基因部分SNP和单倍型与鸭生长和屠体性状的关联性。试验以三水白鸭为材料，利用PCR-RFLP方法检测GHRL基因C-729T、T＋985C和GHSR基因T404C、G3427A共4个SNPs位点的多态性，构建单倍型，进行方差分析。结果表明，C-792T与21、28、35和49日龄体质量、全净膛质量、胸腺质量显著相关（P<0.05），CT基因型杂合子优势明显；T404C与腿肌和脾脏质量显著相关（P<0.05），TT基因型个体均值显著高于CC和TC型个体；G3427A与35日龄体质量、49日龄体质量、屠体质量、半净膛质量、全净膛质量和翅质量显著相关（P <0.05），AA基因型是较为优势的基因型；T+985C与各生长、屠体性状都不相关（P>0.05）。所构建的单倍型与单个SNP的分析结果较为一致，表明这3个SNPs是鸭生长和屠体性状的重要分子标记。

Abstract: The aim of this study was to analyze the association of GHRL and GHSR genes polymorphisms with duck growth and carcass traits. In this study, two SNPs of GHRL gene (C-729T and C+985T) and two SNPs of GHSR gene (T404C and G3427A) were genotyped by PCR-RFLP in a random Sanshui White Duck population to investigate the associations of SNP and their haplotypes with growth and carcass traits. Result showed that C-729T was significantly associated with body weight (BW) at 21 days (BW21), BW28, BW35, BW49, eviscerated weight and Thymus gland weight （P<0.05) Meanwhile, the individuals with CT genotype had better duck body weight gain. T404C was significantly associated with leg muscle weight and spleen weight （P<0.05). And in these traits, the Least-square means of individuals with TT genotype was higher than others. G3427A was significantly associated with BW35, BW49, dressed weight, eviscerated weight with giblet, eviscerated weight and wing weight （P<0.05). And individuals with AA genotype had the highest value of all traits. Otherwise, no significant association of C985T with any growth and carcass traits was found Association analysis of haplotypes indicated similar results with that of SNP It was suggested that C-729T of duck GHRL gene and T404C and G3427A of duck GHSR gene were important molecular markers for duck growth and carcass traits.

方梅霞;李莹;徐海平;谢亮;廖新俤;梁敏;聂庆华;张细权. GHRL及其受体GHSR基因多态性与鸭生长及屠体性状的关联性[J]. 畜牧兽医学报, 2011, 42(1): 18-24.

FANG Mei-xia;;LI Ying;XU Hai-ping;XIE Liang;LIAO Xin-di;LIANG Min;NIE Qing-hua;ZHANG Xi-quan . Associations of Ghrelin (GHRL) and Its Receptor (GHSR) Genes Polymorphismswith Duck Growth and Carcass Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(1): 18-24.

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