Abstract: 2 microsatellite markers OarAE101 and BM143 which were closely linked to the fecundity gene Fec^B in Booroola sheep were analysed for polymorphisms in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat, Boer goat. The number of alleles for OarAE101 was 10, 8, 6 and 8 in 4 goat breeds.The number of alleles for BM143 was 10, 8, 6 and 8 in 4 goat breeds. The polymorphic information contents for OarAE101/BM143 in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat, Boer goat were 0.874 2/0.805 2, 0.796 9/0.809 3, 0.756 4/0.746 2 and 0.796 4/0.734 0, respectively. Least squares means of the litter size for OarAE101 107 bp/111 bp was the best highest (4.0 number born /litter ), the least squares means of genotype 109 bp/109 bp , 107 bp/107 bp , 119 bp /119 bp , 111 bp/111 bp , 125 bp/125 bp for the litter size were higher, was 267, 25, 24, 233 and 225 number born /litter respectively, so the allele 107, 109, 111, 119 and 125 bp for the litter size in Xiangdong black goat had positive effects. Least squares mean of litter size for BM143 100bp/106bp and 106 bp /112 bp for the litter size were 3 number born /litter respectively , there were the positive effects among the allele 104, 106, 110 bp for the litter size of Xiangdong black goat.