Abstract: The halofuginone was modified to prepare Hal-suc as hapten. The hapten was conjugated to BSA as immunogen and to OVA as coated antigen by NHS ester procedure. The anti-Hal serum was acquired in rabbits against the conjugate after sixth immunization. The optional dilution of the anti-Hal serum was 1:102 400 by the indirect ELISA. An ELISA method for the determination of the Hal in chicken liver and muscle tissues was developed. In the chicken liver tissue fortified at 50, 100, 500 ng/g, the average recoveries ranged from 74.2% to 96.8%. In the chicken muscle tissue fortified at 50, 100, 500ng/g, the average recoveries ranged from 74.3% to 90.0%. The detection limits in chicken liver tissue and in chicken muscle tissue were 28ng/g and 19ng/g respectively.