Abstract: The 4 and 8 repeats of CCK33 protein was displayed on phage T4 surface using phage T4 surface display system, and its immunogenicity also was studied. The 4 and 8 repeats of CCK-33 gene were recombined with a SOC gene deleted lysozyme-dependent phage ΦT4-Z1 to yield recombinant phage ΦT-4CCK and ΦT-8CCK. SDS-PAGE analysis result indicated that the recombinant CCK protein was displayed on the surface of phage T4 successfully and their molecular were about 30ku and 41ku, which further tested by Western-Blot demonstrating specific reaction with CCK antibody. The recombinant ΦT-4CCK and ΦT-8CCK were used as an antigen to prepare oilemulsion vaccines. After Hu-xu chicken were immunized with oil-emulsion vaccines, the concentration of anti-serum, the body weight and feed intake of treated group were improved compared with the control group, but the feed meat ratio was decreased. The result showed that the recombinant CCK protein being displayed on phage T4 had good immunogenicity.