Abstract: The aim of this study was to analyze the expression characteristics of chicken Perilipin1 in different tissues and the function of Perilipin1 in lipid metabolism by preparing the antiserum against chicken Perilipin1. Chicken Perilipin1 gene was amplified by RT-PCR and inserted into pET-28a vector. The recombinant His/Perilipin1 was expressed in E-coli with IPTG induction and renatured by Protein Refolding Kit. The renatured His/Perilipin1 was used as immunogen to immunize the rabbit for preparing polyclonal antibody. The titer and specificity of this antibody were detected by ELISA and Western blot, and the expression characteristics of chicken Perilipin1 in different tissues was analyzed by Western blot. ELISA and Western blot results showed that the high titer and specificity of the antiserum against Perilipin1 was obtained The tissue expression characterization analysis by Western blot showed that Perilipin1 only expressed in chicken adipose tissue, but no signal was detected in liver, duodenum, breast muscle, leg muscle, gizzard, heart, spleen and kidney. Furthermore, the Perilipin1 expression characteristics between fat and lean broiler lines were also detected, and the results showed that Perilipin1 expressed significantly higher in fat line broilers adipose tissue than that in lean ones (P<0.05). These results of this study suggest that there is a close relationship between the expression of Perilipin1 and the deposition of abdomen adipose in fat and lean broiler lines.