Abstract: This experiment was conducted to study the genetic variations and phylogenetic analysis of Duck Hepatitis Virus（DHV） in China. The complete nucleotide sequences of 7 isolated strains and 1 vaccine strain of DHV1 had been determined and comparative genome analysis with other DHV strains available in GenBank was performed. The results showed that the VP1 was the most variant gene and hypervariable regions were found in 180194aa and 213219aa of VP1 protein. Variant NLinked glycosylation sites were found in different strains. Phylogenetic and evolutionary analysis showed that our sequencing strains belonged to genotype A, which were on different branches from genotype B and C. Strain JYZ02 had a very close relationship with strain R85952 which suggested that strain JYZ02 was evolved from strain R85952. The results of amino acid sequence identity and virus neutralization test proved that there was no antigenic variation occurred in the seven strains we determined. Phylogenetic analysis revealed that the dominant serotype of epidemic DHV in China was type 1 at present.