TNFα对猪脂肪细胞脂肪分解及其相关基因转录表达的影响

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (9): 1290-1296.doi:

TNFα对猪脂肪细胞脂肪分解及其相关基因转录表达的影响

郑雪莉^1，2 ，李玉成¹ ，鞠大鹏¹ ，杨公社¹*

1.西北农林科技大学动物脂肪沉积与肌肉发育实验室，杨凌 712100； 2. 西北农林科技大学林学院，杨凌 712100

收稿日期:2008-09-10 修回日期:1900-01-01 出版日期:2009-09-24 发布日期:2009-09-24
通讯作者: 杨公社

TNFα Stimulates Lipolysis in Differentiated Porcine Adipocytes and Wownregulated Expression of the Genes Related to Lipolysis

ZHENG Xueli ^1,2, LI Yucheng¹, JU Dapeng ¹, YANG Gongshe¹*

1. Laboratory of Animal Fat Deposition and Muscle Development, Northwest A&F University, Yangling 712100, China; 2. College of Forestry, Northwest A&F University, Yangling 712100, China

Received:2008-09-10 Revised:1900-01-01 Online:2009-09-24 Published:2009-09-24
Contact: YANG Gongshe

摘要/Abstract

摘要：

为探讨TNFα对猪脂肪细胞脂肪分解的调控作用及其机制，用1、10、100 ng·mL1 3种剂量TNFα作用体外培养的猪原代脂肪细胞24 h，及10 ng·mL1 TNFα处理脂肪细胞1、6、12、24、48 h。使用甘油试剂盒，检测细胞甘油的释放量，RTPCR检测ATGL、TGH2、HSL、LPL、MGL和perilipinA表达量变化。结果表明，TNFα以浓度和时间依赖性的方式促进猪原代脂肪细胞甘油的释放(P<0.05)；同时下调ATGL、TGH2、HSL、LPL、MGL和perilipin A mRNA表达量。说明：TNFα主要通过下调perilipin A的表达，降低其对脂滴的保护作用，增加脂肪分解酶和脂滴的接触，促进了猪脂肪细胞的脂解作用。

Abstract:

To study the effect of tumor necrosis factor α(TNFα) on lipolysis in differentiated porcine adipocytes and its mechanism, the differentiated porcine adipocytes were treated with TNFα at the concentrations of 1, 10, 100 ng·mL1 for 24 hours and the concentration of 10 ng·mL1 for 0, 1, 6, 12, 24 and 48 hours , respectively. Commercial kits were used to measure the glycerol release; Semiquantitative RTPCR was used to detect the expression levels of ATGL,TGH2,HSL,LPL,MGL and perilipin A genes. The results showed that TNFα promoted the glycerol release in dose and time dependent manners(P<0.05) in differentiated porcine adipocytes，and downregulated the mRNA expression levels of ATGL, TGH2, HSL, LPL, MGL and perilipin A. These results indicated that TNFα could stimulate lipolysis by downregulating the expression level of the perilipin A which was thought to modulate the ATGL and HSL to the surface of the fat droplet.

郑雪莉;;李玉成;鞠大鹏;杨公社. TNFα对猪脂肪细胞脂肪分解及其相关基因转录表达的影响[J]. 畜牧兽医学报, 2009, 40(9): 1290-1296.

ZHENG Xueli;LI Yucheng;JU Dapeng;YANG Gongshe . TNFα Stimulates Lipolysis in Differentiated Porcine Adipocytes and Wownregulated Expression of the Genes Related to Lipolysis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(9): 1290-1296.

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