Abstract:

The relationship between the developmental ability of buffalo oocytes and the apoptosis of granulose cells was investigated in this study. Granulose cells were analyzed on the apoptosis by Annexin VFITC after the buffalo oocytes were cultured 2224 h in vitro, and which was compared with the maturity and the scoring of oocyte as well as the rate of cleavage and embryos. Results showed that the rate of apoptosis increased with the maturity of oocyte. While with the scoring and developmental capacity of the oocyte decreasing, the rate of apoptosis of granulose cells was increased. The results indicated that the relationship between the developmental capacity of the oocyte and apoptosis of granulose cells was significant. The apoptosis rate of granulose cells could be a developmental potential predictor of the immature oocytes.