Abstract: Insulinlike growth factor 2 has profound effects on the growth and differentiation of animal embryo. Some researches indicated that the normal level of IGF2 have positive correlation with the embryo to youth growth of many large size mammals. This study was designed to investigate the polymorphism of exon 2 of IGF2 gene and its relationship with growth and development in 126 Nanyang cattle by PCRRFLPs with BsrⅠ. The results showed that the frequencies of alleles A and B were 0686 5 and 0313 5 respectively. The allele A was the dominant allele in Nanyang cattle. Individuals with homozygote of allele B had a significantly higher average body weight for born six months and twenty four months age, a most significantly higher average body length and heart girth for all experiment age, a significantly higher average hucklebone width for twelve months, eighteen months and two years age than that of allele A. They also had a significantly higher body length for six month age, heart girth for twelve month age, hucklebone width for eighteen and two year age than that of heterozygote. It was concluded that IGF2 gene is the major gene affecting the growth related traits of Nanyang cattle or it links with the major gene, and the mutation could be used as the molecular genetic marker to select the cattle for faster growth.