Abstract: Ten layingeggyangzhougooses were sacrificed to obtain their follicles, from which granulosa cells were collected. After the granulosa cells were separated, they were fixed by 70% ethanol, and the apoptotic cell death was evaluated by flow cytometry. Meanwhile, the plasma samples were collected to evaluate the level of FSH,LH and E2 by RIA methods. The relationships between hormone contents and cell apoptosis rate were calculated. The results showed that: （1）Whether the follicle was healthy or atresic, the percentage of G1 phase of granulose cells was more than 90%, about 2%-6% granulosa cells were in G2 phase, and the percentage of S phase was the lowest; (2) The apoptosis rate of granulosa cells of atresia follicles was extremely significant higher than that of healthy follicles（P<0.01）; (3) With the incensement of follicular diameter, the apoptosis rate of healthy follicles decreased correspondingly; (4) The level of FSH,E2 of healthy group were higher than that of apoptotic group, while the level of LH was on the other hand.